Cell‐printing and transfer technology applications for bone defects in mice
Cell‐printing and transfer technology applications for bone defects in mice
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DOI：
10.1002/term.366
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发表时间：
2011-10
影响因子：
3.3
通讯作者：
Junichi Tsugawa;M. Komaki;Tomoko Yoshida;K. Nakahama;T. Amagasa;I. Morita
中科院分区：
文献类型：
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作者：
Junichi Tsugawa;M. Komaki;Tomoko Yoshida;K. Nakahama;T. Amagasa;I. Morita
自从多能干细胞被发现以来，基于成骨因子和/或细胞的骨再生治疗近年来受到了极大的关注。我们之前曾报道，利用细胞打印技术在体外构建的毛细血管网络植入可以改善血液灌流。在这里，我们开发了一种新的基板，通过在玻璃表面涂上聚乙二醇膜来创建非粘附性表面，然后进行光刻来微调粘附性，从而实现高效的细胞转移。我们检测了细胞在羊膜上的转移率和小鼠颅骨缺损处的骨再生效率。将KUSA-A1细胞(小鼠成骨细胞)移植到羊膜上1h。与直接细胞接种相比，使用该底物的细胞转移更容易在羊膜上植入细胞。将KUSA-A1细胞移植到羊膜上，用于修复小鼠临界大小的颅骨缺损。微型计算机断层扫描(Micro-CT)分析显示，携带细胞的羊膜快速有效地形成了骨。这些结果表明，细胞打印和转移技术用于制备细胞修饰的羊膜是有利于细胞递送系统的。我们的发现支持开发一种生物稳定和有效的骨再生疗法。版权所有©2011 John Wiley&Sons，Ltd.
Bone regeneration therapy based on the delivery of osteogenic factors and/or cells has received a lot of attention in recent years since the discovery of pluripotent stem cells. We reported previously that the implantation of capillary networks engineered ex vivo by the use of cell‐printing technology could improve blood perfusion. Here, we developed a new substrate prepared by coating glass with polyethylene glycol (PEG) to create a non‐adhesive surface and subsequent photo‐lithography to finely tune the adhesive property for efficient cell transfer. We examined the cell‐transfer efficiency onto amniotic membrane and bone regenerative efficiency in murine calvarial bone defect. Cell transfer of KUSA‐A1 cells (murine osteoblasts) to amniotic membrane was performed for 1 h using the substrates. Cell transfer using the substrate facilitated cell engraftment onto the amniotic membrane compared to that by direct cell inoculation. KUSA‐A1 cells transferred onto the amniotic membrane were applied to critical‐sized calvarial bone defects in mice. Micro‐computed tomography (micro‐CT) analysis showed rapid and effective bone formation by the cell‐equipped amniotic membrane. These results indicate that the cell‐printing and transfer technology used to create the cell‐equipped amniotic membrane was beneficial for the cell delivery system. Our findings support the development of a biologically stable and effective bone regeneration therapy. Copyright © 2011 John Wiley & Sons, Ltd.