Enantioselective effects of cyflumetofen on microbial community and related nitrogen cycle gene function in acid-soil
Enantioselective effects of cyflumetofen on microbial community and related nitrogen cycle gene function in acid-soil
复制标题
氟虫芬对酸性土壤微生物群落及相关氮循环基因功能的对映选择性影响
DOI：
10.1016/j.scitotenv.2020.144831
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发表时间：
2021
影响因子：
9.8
通讯作者：
He Lin
中科院分区：
文献类型：
--
作者：
Shi Linlin;Zhang Ping;He Yuhan;Zeng Fanzhan;Xu Jun;He Lin
丁氟螨酯是一种新型手性杀螨剂，广泛应用于经济作物的螨类防治。CYF在土壤中暴露的环境风险，特别是在对映体水平上，仍然不清楚。我们发现（+）-CYF对映体在酸性土壤中优先降解，导致（−）-CYF富集。16S rRNA和qPCR分析表明，（-）-CYF处理和（+）-CYF处理分别使细菌丰度降低12.79 - 61.80%和2.52 - 52.48%。（−）-CYF处理也降低了多样性。有趣的是，几种有益细菌，例如Alphaproteobacteria（纲）、Sphingomonadaceae（科）和Arthrobacter（种）在（-）-CYF后更加富集。固氮菌的丰度随着时间的推移呈持续下降趋势，（-）-CYF处理的下降幅度为3.24 - 72.94%，（+）-CYF处理的下降幅度为25.37 - 73.11%。与（+）-CYF处理相比，（-）-CYF处理能正向促进硝化作用，而（-）-CYF处理显著降低amoA基因丰度，即对氮循环中的硝化作用产生显著的负向影响。通过我们的进一步研究，发现放线菌、α-变形菌、溶杆菌、鞘氨醇单胞菌、Patescibacteria、假单胞菌和假单胞菌等与HandamoA的氮循环相关基因有协同作用。这些结果有助于在对映体水平上全面评价CYF在酸性土壤中的环境风险。
Cyflumetofen (CYF) is a novel chiral acaricide widely used in commercial crops to control mites. The environmental risks exposed by CYF in the soil, especially at the enantiomer level, remain unclear. We found that the (+)-CYF enantiomer was preferentially degraded in acid-soil, resulting in (−)-CYF enrichment. 16S rRNA and qPCR analysis indicated that decreased bacterial abundance by 12.79–61.80% and 2.52–52.48% in (−)-CYF treatment and (+)-CYF treatment, respectively. Diversity was also decreased with (−)-CYF treatment. Interestingly, several beneficial bacteria, for instance,Alphaproteobacteria(class),Sphingomonadaceae(family), andArthrobacter(specise) were more enriched following (−)-CYF. The abundance of N2-fixing bacteria showed a sustained reduction with time, and the decrease was 3.24–72.94% with (−)-CYF and 25.37–73.11% with (+)-CYF treatment. Compared with the (+)-CYF treatment could positively promote nitrification, while the treatment (−)-CYF significantly reduced the abundance ofamoAgene; namely it significantly negatively affected the nitrification in the nitrogen cycle. Through our further research, we found thatActinobacteria,Alphaproteobacteria,Lysobacter;Sphingomonas,Patescibacteria,Saccharimonadia, andSaccharimonadalesshowed synergistic effects with the nitrogen cycling-related genesnifHandamoA. These results contribute to a comprehensive environmental risk assessment of CYF in acid-soil at the enantiomer level.