Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice
Morphologic and functional analysis of sperm and testes in Aquaporin 7 knockout mice
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DOI：
10.1016/j.fertnstert.2006.07.1522
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发表时间：
2007-03-01
影响因子：
6.7
通讯作者：
Uchida, Shinichi
中科院分区：
文献类型：
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作者：
Sohara, Eisei;Ueda, Otoya;Uchida, Shinichi
目的：探讨水通道蛋白7(AQP7)在睾丸和精子中的功能和形态作用。设计：实验研究。地点：大学和科研院所单位。动物(S)：AQP7基因敲除小鼠(C57BL/6J背景)。干预(S)：非。主要观察指标(S)：睾丸和附睾的形态分析、每日精子生成量、精子活力、体外受精。结果(S)：AQP7基因敲除小鼠与野生型小鼠在睾丸和附睾的形态上没有差异。AQP7基因敲除的雄性小鼠和野生型雄性小鼠的后代数量相似。对AQP7基因敲除小鼠的每日精子生成量和活动率的分析没有显示任何异常。同样，AQP7基因敲除小鼠的精子体外受精率与野生型小鼠无明显差异。结论：雄性AQP7基因敲除小鼠不存在不育现象，其精子形态和功能均无异常。
Objective: To investigate the functional and morphological role of Aquaporin 7 (AQP7) in testis and sperm.Design: Experimental laboratory study.Setting: University and research institute unites.Animal(s): AQP7 knockout mice (C57BL/6J background).Interventions(s): None.Main Outcome Measure(s): Morphologic analysis of testis and epididymis, daily sperm production, sperm motility, in vitro fertilization.Result(s): There was no difference in the morphology of the testes and epididymis between AQP7 knockout and wild-type mice. The AQP7 knockout male mice and wild-type male mice had similar numbers of offspring. Analysis of the daily sperm production and motility of AQP7 knockout mice did not show any abnormalities. Similary, the rate of in vitro fertilization using sperm from AQP7 knockout mice was not different from wild-type mice.Conclusion(s): Male AQP7 knockout mice were not sterile, and their sperm did not show any morpholic and functional abnormalities.