A modified SCID mouse model of HIV infection with utility for testing anti-HIV therapies.
A modified SCID mouse model of HIV infection with utility for testing anti-HIV therapies.
复制标题
改良的 HIV 感染 SCID 小鼠模型，可用于测试抗 HIV 疗法。
DOI：
10.1089/088922203322493076
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Marcotte,TameraK
中科院分区：
文献类型：
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作者：
Pincus,SethH;Fang,Hua;Wilkinson,RoyceA;Olson,WilliamC;Marcotte,TameraK
Using human tumor cells we have developed a mouse model of active HIV infection that may be used for testing antiviral agents, although it does not reflect the pathogenesis of human infection. Irradiated SCID/NOD mice are injected with a tumor of human CD4+lymphoma cells susceptible to infection and at a separate site, tumor cells persistently infected with either primary or T cell line-adapted strains of HIV. The spread of infection from the infected to the susceptible tumor is monitored as plasma p24 and the presence of HIV-infected cells in the spleen. We have used this model to examine the relative efficacy of neutralizing anti-HIV antibodies to halt the spread of infection. We have found that the tetrameric CD4-antibody fusion protein, CD4-IgG2, is highly effective compared to an anti-V3 loop antibody. This animal model, while not replicating the human disease, allows for the simultaneous testing of efficacy, toxicity, and pharmacokinetics of potential new antiviral therapies. The model can easily be powered to enable comparisons between different therapeutic agents and dosing regimens.
