Type II isopentenyl diphosphate isomerase from Synechocystis sp strain PCC 6803
Type II isopentenyl diphosphate isomerase from Synechocystis sp strain PCC 6803
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DOI：
10.1128/jb.186.23.8156-8158.2004
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发表时间：
2004-12-01
影响因子：
3.2
通讯作者：
Poulter, CD
中科院分区：
文献类型：
--
作者：
Barkley, SJ;Desai, SB;Poulter, CD
蓝细菌集胞藻属6803菌株中的开放阅读框sll 1556编码推定的II型异戊烯基二磷酸（IPP）异构酶。在大肠杆菌中产生His（6）-标记的蛋白，并通过Ni 2+层析纯化。同源四聚体酶需要NADPH、黄素单核苷酸和Mg 2+才有活性; K-m（IPP）为52 μ M，k（cat）（IPP）为0.23 s（-1）。
Open reading frame sll1556 in the cyanobacterium Synechocystis sp. strain 6803 encodes a putative type II isopentenyl diphosphate (IPP) isomerase. The His(6)-tagged protein was produced in Escherichia coli and purified by Ni2+ chromatography. The homotetrameric enzyme required NADPH, flavin mononucleotide, and Mg2+ for activity; K-m(IPP) was 52 muM, and k(cat)(IPP) was 0.23 s(-1).