MULTIPLE FORMS OF DYNAMIN ARE ENCODED BY SHIBIRE, A DROSOPHILA GENE INVOLVED IN ENDOCYTOSIS
MULTIPLE FORMS OF DYNAMIN ARE ENCODED BY SHIBIRE, A DROSOPHILA GENE INVOLVED IN ENDOCYTOSIS
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DOI：
10.1038/351583a0
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发表时间：
1991-06-13
期刊：
影响因子：
64.8
通讯作者：
VALLEE, RB
中科院分区：
文献类型：
--
作者：
CHEN, MS;OBAR, RA;VALLEE, RB
DYNAMIN是在牛脑组织中发现的一种相对分子质量为100,000的核苷酸敏感微管结合蛋白。它被发现交联微管成高度有序的束，并在体外微管间滑动中发挥作用。对大鼠脑动力蛋白互补DNA进行克隆和测序，鉴定出约300个氨基酸的n端区域，其中包含gtp结合蛋白的3个共识元件2。该结构域与参与干扰素诱导病毒抗性的哺乳动物Mx蛋白3,4，以及与酿酒酵母VPS1位点的产物广泛同源，VPS1位点在膜蛋白分选5和减数分裂纺锤杆分离6中都有牵连。在培养的哺乳动物细胞的免疫荧光研究中没有观察到含有动力蛋白的微管束7，但有报道称，植物有丝分裂过程中，一种gtp需要蛋白在微管间滑动中起作用8。我们在这里报道，黑腹果蝇含有多种组织特异性和发育调节形式的动力蛋白，这些动力蛋白是shibire位点的产物，先前涉及内噬蛋白分选9,10。
DYNAMIN was discovered in bovine brain tissue as a nucleotide-sensitive microtubule-binding protein of relative molecular mass 100,000 1. It was found to cross-link microtubules into highly ordered bundles, and appeared to have a role in intermicrotubule sliding in vitro. Cloning and sequencing of rat brain dynamin complementary DNA identified an N-terminal region of about 300 amino acids which contained the three consensus elements characteristic of GTP-binding proteins 2. Extensive homology was found between this domain and the mammalian Mx proteins which are involved in interferon-induced viral resistance 3,4, and with the product of the VPS1 locus in Saccharomyces cerevisiae, which has been implicated both in membrane protein sorting 5, and in meiotic spindle pole separation 6. Dynamin-containing microtubule bundles were not observed in an immunofluorescence study of cultured mammalian cells 7, but a role for a GTP-requiring protein in intermicrotubule sliding during mitosis in plants has been reported 8. We report here that Drosophila melanogaster contains multiple tissue-specific and developmentally-regulated forms of dynamin, which are products of the shibire locus previously implicated in endocytic protein sorting 9,10.