Nonthermal irreversible electroporation for intracranial surgical applications Laboratory investigation
Nonthermal irreversible electroporation for intracranial surgical applications Laboratory investigation
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DOI：
10.3171/2010.5.jns091448
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发表时间：
2011-03-01
影响因子：
4.1
通讯作者：
Davalos, Rafael V.
中科院分区：
文献类型：
--
作者：
Ellis, Thomas L.;Garcia, Paulo A.;Davalos, Rafael V.
目的。非热不可逆电穿孔（NTIRE）是一种治疗癌症的新型微创技术，其独特之处在于其非热肿瘤消融机制。本文评估了 NTIRE 手术对正常犬脑组织损伤的安全性。方法。 WIRE 手术包括将电极放入 3 只狗的大脑目标区域，并发出一系列短而强的电脉冲。施加的脉冲电压因狗而异。另一只狗用作假对照。在极端电压下处理另外一个堵塞物，以确定该过程的安全上限。手术时使用超声检查来确定术中是否可以看到病变。然后通过术后 MR 成像估计消融组织的体积。然后分析组织学脑切片以评估产生的损伤。结果。除了在上限电压下治疗的动物外，动物耐受该过程，没有明显的并发症。损伤体积似乎随着所施加的脉冲电压的降低而减小。组织学检查显示处理体积内的细胞死亡，坏死脑和正常脑之间有亚毫米过渡区。结论。作者的结果表明，选定电压下的 NTIRE 可以安全地在正常犬脑中进行，并且消融组织的体积与所施加脉冲的电压相关。这项初步研究是使用 NTIRE 作为脑癌治疗的第一步。 (DOI:10.3171/2010.5.JNS091448)
Object. Nonthermal irreversible electroporation (NTIRE) is a novel, minimally invasive technique to treat cancer, which is unique because of its nonthermal mechanism of tumor ablation. This paper evaluates the safety of an NTIRE procedure to lesion normal canine brain tissue.Methods. The WIRE procedure involved placing electrodes into a targeted area of brain in 3 dogs and delivering a series of short and intense electric pulses. The voltages of the pulses applied were varied between dogs. Another dog was used as a sham control. One additional clog was treated at an extreme voltage to determine the upper safety limits of the procedure. Ultrasonography was used at the time of the procedure to determine if the lesions could be visualized intraoperatively. The volumes of ablated tissue were then estimated on postprocedure MR imaging. Histological brain sections were then analyzed to evaluate the lesions produced.Results. The animals tolerated the procedure with no apparent complications except for the animal that was treated at the upper voltage limit. The lesion volume appeared to decrease with decreasing voltage of applied pulses. Histological examination revealed cell death within the treated volume with a submillimeter transition zone between necrotic and normal brain.Conclusions. The authors' results reveal that NTIRE at selected voltages can be safely administered in normal canine brain and that the volume of ablated tissue correlates with the voltage of the applied pulses. This preliminary study is the first step toward using NTIRE as a brain cancer treatment. (DOI:10.3171/2010.5.JNS091448)