Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander.
Alginate lyases in the hepatopancreas of a marine mollusc, Dolabella auricula Solander.
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海洋软体动物 Dolabella auricula Solander 肝胰腺中的藻酸盐裂解酶。
DOI：
10.1093/oxfordjournals.jbchem.a128859
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发表时间：
1968
影响因子：
2.7
通讯作者：
Y. Kashiwabara
中科院分区：
文献类型：
--
作者：
K. Nisizawa;S. Fujibayashi;Y. Kashiwabara
从海洋软体动物Dolabella auriculaSolander的肝前体中分离得到两种多聚甘露糖苷酸裂解酶组分。这些裂解酶对富含甘露糖醛酸的藻酸盐和短链聚甘露糖醛酸苷表现出高活性，但对短链聚古洛糖醛酸苷则没有。这些裂解酶对各种褐藻的藻酸盐的降解是不完全的，其降解程度似乎与其甘露糖醛酸含量大致成正比。从这些底物中，裂解酶主要产生两种二糖醛酸苷和一种三糖醛酸苷，但不产生游离糖醛酸。根据各种结构分析，初步鉴定这些寡糖为β-甘露双糖醛酸、β-甘露双糖醛酸的含双键衍生物和β-甘露三糖醛酸的含双键衍生物。双键似乎位于非还原性末端残基的C4和C5碳原子之间。
Two enzyme components of polymannuronide lyase were obtained from the hepatopancereas of a marine mollusc,Dolabella auriculaSolander. These lyases showed high activities toward mannuronic acid-rich alginate and a short-chain polymannuronide, but not toward a short-chain polyguluronide. The degradation of alginates from various brown algae by these lyases was incomplete and the extent of their degradation seemed roughly proportional to their mannuronic acid content. From these substrates, the lyases mainly produced two kinds of diuronides and a triuronide, but no free uronic acid. On the basis of various structural analyses, these oligo-uronides were tentatively identified as a β-mannobiuronic acid, a derivative of β-mannobiuronic acid containing a double bond, and a derivative of β-mannotriuronic acid containing a double bond. The double bond seemed to be located between the C4and C5carbon atoms of the nonreducing end residue.