Micro-CT evaluation of in vivo osteogenesis at implants processed by wire-type electric discharge machining.
Micro-CT evaluation of in vivo osteogenesis at implants processed by wire-type electric discharge machining.
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DOI：
10.4012/dmj.2011-051
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发表时间：
2012-05
影响因子：
2.5
通讯作者：
Koichi Yamaki;Y. Kataoka;Fukunaga Ohtsuka;T. Miyazaki
中科院分区：
文献类型：
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作者：
Koichi Yamaki;Y. Kataoka;Fukunaga Ohtsuka;T. Miyazaki
电火花线切割钛表面是一种具有不规则形貌的微细加工表面，具有良好的体外骨生物相容性。在这项研究中，在EDM表面上的体内成骨的效率进行了调查，通过手术将螺丝形的EDM加工和加工表面植入到四个日本白色兔的股骨。在植入后1、2和4周，通过X射线显微CT扫描仪评价新骨形成的体积和过程，并结合组织病理学观察。在手术植入前，检查每个植入物表面的表面形貌和接触角。两种种植体表面的骨形成随时间推移而增加，两种种植体类型在植入后4周产生的骨体积在统计学上相等。然而，在植入后1周，EDM处理的种植体的新骨量明显大于机加工表面种植体。此外，新骨似乎直接从EDM表面开始，而新骨似乎从预先存在的宿主骨生成到机加工表面。因此，电火花加工似乎是一种很有前途的方法，表面改性钛种植体，以支持增强成骨。
Titanium surfaces processed by wire-type electric discharge machining (EDM) are microfabricated surfaces with an irregular morphology, and they exhibited excellent in vitro bone biocompatibility. In this study, the efficiency of in vivo osteogenesis on EDM surfaces was investigated by surgically placing screw-shaped EDM-processed and machined-surface implants into the femurs of four Japanese white rabbits. The volume and process of new bone formation were evaluated by an X-ray micro-CT scanner, coupled with histopathological observations at 1, 2, and 4 weeks post-implantation. Before surgical implantation, the surface topography and contact angle of each implant surface were examined. Bone formation increased over time on both implant surfaces, with both implant types yielding statistically equivalent bone volume at 4 weeks post-implementation. However, at 1 week post-implantation, amount of new bone at EDM-processed implant was markedly greater than that at machined-surface implant. Moreover, new bone appeared to initiate directly from the EDM surfaces, while new bone appeared to generate from pre-existing host bone to the machined surfaces. Thus, EDM seemed to be a promising method for surface modification of titanium implants to support enhanced osteogenesis.