Effect of embryonic treatment with oestradiol benzoate on reproductive morphology, ovulation and oviposition and plasma LH concentrations in female quail (Coturnix coturnix japonica).
Effect of embryonic treatment with oestradiol benzoate on reproductive morphology, ovulation and oviposition and plasma LH concentrations in female quail (Coturnix coturnix japonica).
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苯甲酸雌二醇胚胎处理对雌性鹌鹑 (Coturnix coturnix japonica) 生殖形态、排卵和产卵以及血浆 LH 浓度的影响。
DOI：
10.1530/jrf.0.0710411
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
E. Adkins
中科院分区：
文献类型：
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作者：
E. Rissman;M. Ascenzi;P. Johnson;E. Adkins
鹌鹑蛋孵育第10天分别注射0、5、10、20、40微克雌二醇苯甲酸酯。在16L: 8D光周期上饲养这些卵孵化的雌性，并记录产卵情况。在37、40、43、46、49、52、55、58或61日龄时采集血样，采用双抗体放射免疫法测定LH浓度。61日龄时扑杀；对卵巢和输卵管进行称重和检查。雌二醇苯甲酸酯处理大大减少了产卵量，但对于产卵的鸟类，第一次产卵的年龄是正常的。黄体生成素水平不受苯甲酸雌二醇治疗的影响，在40和49日龄时最高。苯甲酸雌二醇对卵巢重量、直径大于1cm的卵泡数、排卵后卵泡数无影响。雌二醇苯甲酸酯对雌性拥有两条输卵管的可能性有剂量相关的影响，对于那些保留了右侧输卵管的雌性来说，左侧输卵管比正常情况下要小。经苯甲酸雌二醇处理的雌性更有可能在体腔内排出卵黄。因此，用苯甲酸雌二醇处理胚胎似乎是通过引起输卵管异常来抑制产卵，而不是通过（在哺乳动物中发生的）抑制排卵。
Quail eggs were injected on Day 10 of incubation with 0, 5, 10, 20 or 40 micrograms oestradiol benzoate. Females hatching from these eggs were reared on a 16L: 8D photoperiod and egg laying was recorded. Blood samples were taken at 37, 40, 43, 46, 49, 52, 55, 58 or 61 days of age and LH concentrations were measured by a double-antibody radioimmunoassay. Birds were killed at 61 days of age; ovaries and oviducts were weighed and examined. Egg laying was greatly reduced by oestradiol benzoate treatment, but for birds that did lay, age at first oviposition was normal. LH levels were not affected by oestradiol benzoate treatment, and were highest at 40 and 49 days of age. Oestradiol benzoate had no effect on ovarian weight, number of follicles with diameter greater than 1 cm, or number of post-ovulatory follicles. Oestradiol benzoate had a dose-related effect on the likelihood that females would have two oviducts, and for those females that had retained the right oviduct, the left oviduct was smaller than normal. Oestradiol benzoate-treated females were more likely to have ovulated yolks in the body cavity. Embryonic treatment with oestradiol benzoate therefore appears to inhibit egg laying by causing oviduct abnormalities, rather than by (as happens in mammals) inhibiting ovulation.