Spatial diversity in gene expression for VDCCγ subunit family in developing and adult mouse brains
Spatial diversity in gene expression for VDCCγ subunit family in developing and adult mouse brains
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DOI：
10.1016/j.neures.2005.08.009
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发表时间：
2005-12-01
影响因子：
2.9
通讯作者：
Watanabe, M
中科院分区：
文献类型：
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作者：
Fukaya, M;Yamazaki, M;Watanabe, M
电压依赖性钙通道（VDCCs）的γ亚基的特征在于AMPA型谷氨酸受体和VDCCs的分子多样性和调节。在本研究中，我们研究了VDCC γ 1 -8亚基mRNA在发育和成年小鼠大脑原位杂交的表达。在成人脑中，γ 2和γ 7亚基mRNA广泛表达于各种灰质区域，其中小脑浦肯野细胞和颗粒细胞中的表达水平最高。γ 3和γ 8亚基mRNA在端脑中占主导地位，后者在海马中处于惊人的水平。γ 4亚基mRNA在嗅球、纹状体、丘脑和下丘脑中富集。γ 5亚基mRNA在嗅球、海马CA 2区、丘脑、下丘和Bergmann胶质细胞中含量丰富。在胚胎大脑中检测到这些亚基的转录本：一些显示出保存良好的空间模式（γ 2，γ 5，γ 7和γ 8），而另一些则经历了发育上调（γ 3）或下调（γ 4）。相反，γ 1和γ 6亚基mRNA在整个大脑发育过程中呈阴性或非常低。因此，本研究揭示了单个VDCC γ亚基基因表达的空间多样性，可能反映了该蛋白质家族的功能多样性及其在神经功能中的差异参与。(c)2005年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
The gamma subunit of voltage-dependent Ca2+ channels (VDCCs) is characterized by molecular diversity and regulation of AMPA-type glutamate receptors as well as VDCCs. In the present study, we examined expressions for the VDCC gamma l-8 subunit mRNAs in developing and adult mouse brains by in situ hybridization. In adult brains, the gamma 2 and gamma 7 subunit mRNAs were widely expressed in various grey matter regions with the highest level in cerebellar Purkinje cells and granule cells. The gamma 3 and gamma 8 subunit mRNAs predominated in the telencephalon, with the latter being at striking levels in the hippocampus. The gamma 4 subunit mRNA was enriched in the olfactory bulb, striatum, thalamus and hypothalamus. The gamma 5 subunit mRNA was abundant in the olfactory bulb, hippocampal CA2, thalamus, inferior colliculus and Bergmann glia. Transcripts of these subunits were detected in embryonic brains: some showed well-preserved spatial patterns (gamma 2, gamma 5, gamma 7 and gamma 8), while others underwent developmental up- (gamma 3) or down-regulation (gamma 4). In contrast, the gamma 1 and gamma 6 subunit mRNAs were negative or very low throughout brain development. Therefore, the present study has revealed spatial diversity in gene expression for individual VDCC gamma subunits, presumably reflecting functional diversity of this protein family and their differential involvement in neural function. (c) 2005 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.