Thyrocyte-specific expression of Cre recombinase in transgenic mice
Thyrocyte-specific expression of Cre recombinase in transgenic mice
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DOI：
10.1002/gene.20043
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发表时间：
2004-07-01
期刊：
影响因子：
1.5
通讯作者：
Kimura, S
中科院分区：
文献类型：
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作者：
Kusakabe, T;Kawaguchi, A;Kimura, S
建立了人甲状腺过氧化物酶(TPO)基因启动子控制下表达Cre重组酶的转基因小鼠系。用Northern印迹和与rosa26报告基因转基因小鼠杂交的方法检测了TPO驱动的Cre重组酶的活性和特异性。在后一组小鼠中，Cre介导的重组仅发生在甲状腺细胞中，重组在胚胎14.5天左右开始，此时是甲状腺器官发生期间TPO开始表达的时间。本研究表明，TPO-Cre转基因小鼠是特异性删除甲状腺细胞中loxP插入(FLOXED)基因的有力工具，将在甲状腺细胞发育和(或)成体甲状腺中特异基因的研究中具有重要价值。(C)2004年Wiley-Liss公司
A transgenic mouse line that expresses Cre recombinase under control of the human thyroid peroxidase (TPO) gene promoter was established. The activity and specificity of the TPO-driven Cre recombinase were examined by using Northern blotting and by crossing with the ROSA26 reporter transgenic mouse line. In the latter mice, Cre-mediated recombination occurred only in the thyrocytes, and recombination commenced around embryonic day 14.5, at the time during thyroid organogenesis when TPO expression begins. This study demonstrates that the TPO-Cre transgenic mouse is a powerful tool to specifically delete loxP-inserted (floxed) genes in thyrocytes and will be of great value in the study of thyrocyte-specific genes during development and/or in adult thyroids. (C) 2004 Wiley-Liss, Inc.