Isolation and basic characterization of temperature-sensitive mutants from Semliki Forest virus;.
Isolation and basic characterization of temperature-sensitive mutants from Semliki Forest virus;.
复制标题
塞姆利基森林病毒温度敏感突变体的分离和基本特征；。
DOI：
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
L. Kääriäinen
中科院分区：
文献类型：
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作者：
S. Keränen;L. Kääriäinen
从n -甲基-n '-硝基-n -亚硝基胍处理的塞姆利基森林病毒中分离出16个温度敏感突变体。其中7个突变体在非允许温度（39℃）下不能合成病毒特异性RNA，被归类为RNA突变体；温度升高实验证明，它们只是在早期功能上有缺陷。两个被指定为RNA±的突变体比野生型病毒产生更少的病毒特异性RNA。6个RNA+突变体在39°C下合成了42 S和26 S RNA，其数量和比例与野生型几乎相同。其中只有ts-3在非允许温度下不能形成病毒140s核衣壳。其中一个RNA+突变体ts-1在27°C和39°C下产生了更高比例的42 S RNA，可能是以26 S RNA为代价的。在Semliki Forest和Sindbis野生型病毒感染细胞中，42 S RNA和26 S RNA的合成是温度依赖性的。在39°C时，42 S RNA的合成比26 S RNA的合成增加，在27°C时情况正好相反。
Sixteen temperature-sensitive mutants were isolated from N-methyl-N'-nitro-N-nitrosoguanidine treated Semliki Forest virus. Seven of the mutants were unable to synthesize virus specific RNA at the nonpermissive temperature (39° C) and were classified as RNA mutants; as evidenced by temperature shift-up experiments they had defects only in early functions. Two mutants, designated RNA ±, made less virus specific RNA than the wild type virus. Six RNA+ mutants synthesized 42 S and 26 S RNA at 39° C in almost the same amounts and ratio as the wild type. Only one of them, ts-3, was unable to form the viral 140 S nucleocapsid at the nonpermissive temperature. One of the RNA+ mutants, ts-1, made a higher proportion of 42 S RNA, presumably at the expense of 26 S RNA, both at 27° C and at 39° C. The synthesis of 42 S RNA and 26 S RNA in the Semliki Forest and Sindbis wild type virus infected cells was temperature dependent. There was an increased synthesis of 42 S RNA compared to 26 S RNA at 39° C, the situation being reversed at 27° C.