Transgenic Expression of the Functional Fragment Hpa110-42 of the Harpin Protein Hpa1 Imparts Enhanced Resistance to Powdery Mildew in Wheat.
Transgenic Expression of the Functional Fragment Hpa110-42 of the Harpin Protein Hpa1 Imparts Enhanced Resistance to Powdery Mildew in Wheat.
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DOI：
10.1094/pdis-07-13-0687-re
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发表时间：
2014-03
期刊：
影响因子：
4.5
通讯作者：
Defu Wang;Yajun Wang;M. Fu;Shuyuan Mu;B. Han;Hongtao Ji;Hongsheng Cai;Hansong Dong;Chunling Zhang-Ch
中科院分区：
文献类型：
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作者：
Defu Wang;Yajun Wang;M. Fu;Shuyuan Mu;B. Han;Hongtao Ji;Hongsheng Cai;Hansong Dong;Chunling Zhang-Ch
小麦白粉病是世界性的小麦毁灭性病害之一，由小麦白粉病菌引起。真菌是一种种群数量不断变化的真菌，这往往对具有宿主抗性的疾病管理构成挑战。利用广谱抗性的转基因方法可以限制病原体种群的变化，并有助于有效控制疾病。由水稻白叶枯病菌产生的Harpin蛋白Hpa 1能诱导水稻对白叶枯病和稻瘟病的抗性。据报道，包含Hpa 1的残基10至42的片段Hpa 110 -42比全长蛋白有效3至8倍。本研究利用小麦抗白粉病基因Hpa 110 -42进行转基因表达。禾本科植物白藓获得了9个Hpa 110 -42转基因小麦品系。证实了Hpa 110 -42的基因组整合，并且在各个转基因系中检测到不同水平的转基因表达。接种E.禾本科植物结果表明，转基因株系对白粉病的抗性显著高于未转基因株系。因此，Hpa 110 -42的转基因表达赋予了对一个E.禾本科植物在温室条件下，小麦中的小麦赤霉病菌
Powdery mildew, one of devastating diseases of wheat worldwide, is caused by Erysiphe graminis f. sp. tritici, a fungal species with constant population changes, which often poses challenges in disease management with host resistance. Transgenic approaches that utilize broad-spectrum resistance may limit changes of pathogen populations and contribute to effective control of the disease. The harpin protein Hpa1, produced by the rice bacterial blight pathogen, can induce resistance to bacterial blight and blast in rice. The fragment comprising residues 10 through 42 of Hpa1, Hpa110-42, is reportedly three- to eightfold more effective than the full-length protein. This study evaluated the transgenic expression of the Hpa110-42 gene for resistance to powdery mildew in wheat caused by E. graminis f. sp. tritici. Nine Hpa110-42 transgenic wheat lines were generated. The genomic integration of Hpa110-42 was confirmed, and expression of the transgene was detected at different levels in the individual transgenic lines. Following inoculation with the E. graminis f. sp. tritici isolate Egt15 in the greenhouse, five transgenic lines had significantly higher levels of resistance to powdery mildew compared with nontransformed plants. Thus, transgenic expression of Hpa110-42 conferred resistance to one isolate of E. graminis f. sp. tritici in wheat in the greenhouse.