Separation and comparison of two monokines with lymphocyte-activating factor activity: IL-1 beta and hybridoma growth factor (HGF). Identification of leukocyte-derived HGF as IL-6.
Separation and comparison of two monokines with lymphocyte-activating factor activity: IL-1 beta and hybridoma growth factor (HGF). Identification of leukocyte-derived HGF as IL-6.
复制标题
分离和比较两种具有淋巴细胞激活因子活性的单核因子：IL-1β 和杂交瘤生长因子 (HGF)。
作者：
J. Damme;J. V. Beeumen;B. Decock;J. Snick;M. Ley;A. Billiau
有丝分裂原刺激的人白细胞的上清液含有两种生物学相关的细胞因子，IL-1和杂交瘤生长因子（HGF）。IL-1 β是成纤维细胞中HGF的有效诱导剂，但对自发产生HGF的单核细胞几乎没有刺激作用。白细胞衍生的HGF和IL-1通过使用特异性抗体上的亲和层析和两种细胞因子的鉴别测定系统分离。经凝胶过滤和SDS-PAGE分析，它们的Mr值不同。与IL-1 β相反，HGF在阳离子交换柱上显示出异质性。IL-1 β和HGF分别通过四个和五个纯化步骤的序列纯化至同质。白细胞衍生的HGF通过其NH 2-末端氨基酸序列的分析来表征。这揭示了与成纤维细胞衍生的HGF、26-kDa蛋白、IFN-β 2和B细胞刺激因子2（统称为IL-6的分子）完全同源。IL-1 β对成纤维细胞具有抗病毒和促生长作用，而HGF/IL-6则无此作用。IL-1和IL-6均具有淋巴细胞活化因子活性，仅能被相应细胞因子的抗血清中和。
Supernatants of mitogen-stimulated human leukocytes contain two biologically related cytokines, IL-1 and hybridoma growth factor (HGF). IL-1 beta is a potent inducer of HGF in fibroblasts but has little stimulating effect on monocytes that spontaneously produce HGF. Leukocyte-derived HGF and IL-1 were separated by the use of affinity chromatography on specific antibodies and discriminating assay systems for both cytokines. They had different Mr upon gel filtration and SDS-PAGE. In contrast to IL-1 beta, HGF showed heterogeneity on a cation-exchange column. IL-1 beta and HGF were purified to homogeneity by a sequence of four and five purification steps, respectively. Leukocyte-derived HGF was characterized by analysis of its NH2-terminal amino acid sequence. This revealed complete homology with fibroblast-derived HGF, 26-kDa protein, IFN-beta 2, and B cell stimulatory factor 2, molecules which have collectively been designated as IL-6. IL-1 beta exerted an antiviral and growth-promoting effect of fibroblasts, whereas HGF/IL-6 did not. Both IL-1 and IL-6 possessed lymphocyte-activating factor activity, which could be neutralized only by an anti-serum against the corresponding cytokine.