A grinding method for producing sections of large areas of plastic embedded tissues
A grinding method for producing sections of large areas of plastic embedded tissues
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一种大面积塑料包埋组织切片的研磨方法
DOI：
10.1111/j.1365-2818.1982.tb00422.x
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发表时间：
1982
影响因子：
2
通讯作者：
A. Zaki
中科院分区：
文献类型：
--
作者：
D. Weber;D. Eisenmann;A. Zaki
我们开发了一种方法，利用相对较厚的塑料包埋组织的研磨切片，提供了0.5 μm切片获得的分辨率。这些切片永久固定在塑料显微镜载玻片上，面积比超薄切片机切片大得多。其他优点是：切片可以脱色和再染色，并且可以用各种形式的电子显微镜检查选定的区域。放射自显影研究也是可能的。虽然该方法具有更广泛的应用，但它在检查硬组织和软组织之间的界面时特别有用。
We have developed a method utilizing relatively thick ground sections of plastic embedded tissue which affords the resolution obtained with 0·5 μm cut sections. The sections, which are permanently affixed to plastic microscope slides, are much larger in area than ultramicrotome sections. Additional advantages are: sections can be destained and restained and selected areas can be examined with various forms of electron microscopy. Autoradiographic studies are also possible. Although the method has a broader application, it is particularly useful in examining the interface between hard and soft tissues.