Regulation and developmental expression of the divalent metal-ion transporter in the rat brain.
Regulation and developmental expression of the divalent metal-ion transporter in the rat brain.
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发表时间：
2000-05
影响因子：
1.6
通讯作者：
K. Williams;M. A. Wilson;J. Bressler
中科院分区：
文献类型：
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作者：
K. Williams;M. A. Wilson;J. Bressler
二价金属离子转运体1(DMT1)是最近发现的一种金属离子转运体，它似乎介导了肠道对铁的吸收。DMT1mRNA也存在于大脑的不连续区域。在本研究中，我们检测了DMT1mRNA在发育中的大鼠脑中的表达。原位杂交发现DMT1在纹状体、皮质、海马区和小脑中均有表达。在发育过程中，出生后14天(PND)和30天(PND)的浦肯野细胞和小脑颗粒细胞中均有DMT1mRNA的表达。在出生后14天，DMT1基因在小脑外颗粒层也有表达。Northern分析显示，在PND 1~21岁不同年龄的小脑中，DMT1基因的表达水平没有变化。Northern分析表明，在培养的大鼠星形胶质细胞中发现了DMT1。蛋白激酶C的激活增加了DMT1在新生大鼠肾上皮细胞中的表达，但不能增加星形胶质细胞的表达。由于DMT1在各种类型的细胞中都有表达，我们认为它在大脑中的金属动态平衡中发挥着重要作用。
Divalent metal ion transporter 1 (DMT1) is a recently identified metal-ion transporter that appears to mediate the absorption of iron in the intestine. DMT1 mRNA is also present in discrete areas of the brain. In this study, we examined the expression of DMT1 mRNA in developing rat brain. DMT1 mRNA was found by in situ hybridization in the striatum, cortex, hippocampus and cerebellum. During development, DMT1 mRNA was found in Purkinje and granule cells in the cerebellum at post-natal day (PND) 14 and PND 30. DMT1 mRNA was also expressed in the external granular layer of the cerebellum at PND 14. No change in the level of DMT1 mRNA was observed by Northern analysis in the cerebellum at different ages between PND 1 and 21. DMT1 was found by Northern analysis in cultures of rat astrocytes. Activation of protein kinase C increased the expression of DMT1 in kidney epithelial cells but not astrocytes from newborn rats. Because DMT1 is expressed in a wide variety of types of cells, we suggest that it plays an important role in metal homeostasis in the brain.