A NEUROPEPTIDE GENE DEFINED BY THE DROSOPHILA MEMORY MUTANT AMNESIAC
A NEUROPEPTIDE GENE DEFINED BY THE DROSOPHILA MEMORY MUTANT AMNESIAC
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DOI：
10.1126/science.7754370
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发表时间：
1995-05-12
期刊：
影响因子：
56.9
通讯作者：
QUINN, WG
中科院分区：
文献类型：
--
作者：
FEANY, MB;QUINN, WG
果蝇的联想学习和记忆所需的基因突变是存在的，但这些基因的分离一直很困难，因为大多数基因是由一个单一的化学诱导的等位基因定义的。在这里，一种简化的基因筛选被用来识别与学习和记忆有关的候选基因。利用转座子诱变技术分离了dnc雌性不育表型的第二位点抑制子。其中一个抑制基因突变在失忆症（amn）基因中被发现。基因座的克隆表明，人类编码一个以前未被表征的神经肽基因。因此，随着amn的克隆，特定的神经肽与记忆过程有关。
Mutations in genes required for associative learning and memory in Drosophila exist, but isolation of the genes has been difficult because most are defined by a single, chemically induced allele. Here, a simplified genetic screen was used to identify candidate genes involved in learning and memory. Second site suppressors of the dunce (dnc) female sterility phenotype were isolated with the use of transposon mutagenesis. One suppressor mutation that was recovered mapped in the amnesiac (amn) gene. Cloning of the locus revealed that amn encodes a previously uncharacterized neuropeptide gene. Thus, with the cloning of amn, specific neuropeptides are implicated in the memory process.