Importin α/β-mediated nuclear protein import is regulated in a cell cycle-dependent manner
Importin α/β-mediated nuclear protein import is regulated in a cell cycle-dependent manner
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DOI：
10.1016/j.yexcr.2004.03.010
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发表时间：
2004-07-01
影响因子：
3.7
通讯作者：
Yoneda, Y
中科院分区：
文献类型：
--
作者：
Yasuhara, N;Takeda, E;Yoneda, Y
功能性核蛋白通过转运因子如进口蛋白α和β选择性导入细胞核。使用特定的输入底物来测量输入α / β介导途径的核蛋白输入效率与细胞周期之间的关系。在细胞周期的某一阶段，将含有SV40 T抗原核定位信号（nuclear localization signal， NLS）的底物微量注射到同步培养细胞的细胞质中，动态测量底物的核输入。发现进口效率的变化依赖于细胞周期，而不是容量。即前期S期、G2/M期和M/G1期进口效率较低。此外，我们发现细胞提取物中输入蛋白α和输入蛋白β的共免疫沉淀程度与输入效率密切相关。这些结果表明，输入蛋白α / β介导的核输入机制通过调节输入蛋白α和β之间的相互作用模式，以细胞周期依赖的方式进行调节。(C) 2004爱思唯尔公司版权所有。
Functional nuclear proteins are selectively imported into the nucleus by transport factors such as importins alpha and beta. The relationship between the efficiency of nuclear protein import and the cell cycle was measured using specific import substrates for the importin alpha/beta-mediated pathway. After the microinjection of SV40 T antigen nuclear localization signal (NLS)-containing substrates into the cytoplasm of synchronized culture cells at a certain phase of the cell cycle, the nuclear import of the substrates was measured kinetically. Cell cycle-dependent change in import efficiency, but not capacity, was found. That is, import efficiency was found low in the early S, G2/M, and M/G1 phases compared with other phases. In addition, we found that the extent of co-imunoprecipitation of importin alpha with importin beta from cell extracts was strongly associated with import efficiency. These results indicate that the importin alpha/beta-mediated nuclear import machinery is regulated in a cell cycle-dependent manner through the modulation of interaction modes between importins alpha and beta. (C) 2004 Elsevier Inc. All rights reserved.