Three-dimensional measurement of ice crystals in frozen beef with a micro-slicer image processing system
Three-dimensional measurement of ice crystals in frozen beef with a micro-slicer image processing system
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DOI：
10.1016/s0140-7007(03)00042-2
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发表时间：
2004-03
期刊：
影响因子：
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通讯作者：
G. Do;Y. Sagara;Mizuho Tabata;K. Kudoh;T. Higuchi
中科院分区：
文献类型：
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作者：
G. Do;Y. Sagara;Mizuho Tabata;K. Kudoh;T. Higuchi
研究了一种利用微切片图像处理系统（MSIPS）测量冷冻牛肉中冰晶三维结构和分布的新技术。该系统具有对最小厚度为1 μm的冷冻样品进行多次切片所获得的暴露截面图像数据进行三维图像重建的功能，并能显示样品的内部结构和任意截面的观察角度。冰晶的大小和分布可以从晶体的外围和面积等二维定量信息中确定。通过荧光指示剂对生牛肉的染色观察，定量地论证了冷冻条件对冰晶形态和分布的影响。在- 15°C条件下，冰晶的网络结构主要分布在细胞间隙，截面尺寸约为100 μm，而在- 120°C条件下，冰晶在细胞内的直径为10 ~ 20 μm。样品的三维图像表明，冰柱的生长受到肌肉纤维固有结构的限制。该方法为研究冻结条件对冰晶大小、形态和分布的影响提供了一种新的工具。
A novel technique has been developed for measuring the three-dimensional (3-D) structure and distribution of ice crystals formed in frozen beef by using a micro-slicer image processing system (MSIPS). The system has functions to reconstruct the 3-D image based on the image data of exposed cross-sections obtained by multi-slicing of a frozen sample with the minimum thickness of 1 μm and to display the internal structure as well as an arbitrary cross-section of the sample choosing observation angles. The size and distribution of ice crystals can be determined from the 2-D quantitative information, such as the periphery and area of the crystals. The effects of freezing conditions on the morphology and distribution of the ice crystals were demonstrated quantitatively from the observations of raw beef stained by fluorescent indicator. For the samples frozen at −15 °C, the network structure of ice crystals were observed mainly at intercellular space, having approximately 100 μm in cross-sectional size, while that prepared at −120 °C showed the spherical crystals of 10–20 μm in diameter within the cells. The 3-D image of the sample demonstrated that the growth of ice columns was restricted by the intrinsic structure of muscle fibers. The proposed method provided a new tool to investigate the effects of freezing conditions on the size, morphology and distribution of ice crystals.