Localization of cellular retinol-binding protein and cellular retinoic acid-binding protein in the rat testis and epididymis.
Localization of cellular retinol-binding protein and cellular retinoic acid-binding protein in the rat testis and epididymis.
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细胞视黄醇结合蛋白和细胞视黄酸结合蛋白在大鼠睾丸和附睾中的定位。
DOI：
10.1002/j.1939-4640.1985.tb00836.x
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发表时间：
1985
影响因子：
--
通讯作者：
Orgebin-Crist,MC
中科院分区：
文献类型：
--
作者：
Porter,SB;Ong,DE;Chytil,F;Orgebin-Crist,MC
用过氧化物酶-抗过氧化物酶免疫定位技术研究了细胞视黄醇结合蛋白（CRBP）和细胞视黄酸结合蛋白（CRABP）在大鼠睾丸和附睾中的分布。在睾丸中，细胞视黄醇结合蛋白仅定位于支持细胞中。染色随生精上皮周期的阶段而变化，并且在成熟分裂之前最大。细胞视黄酸结合蛋白仅定位于近腔室的生发细胞中。结果表明，视黄酸可能是由生发细胞使用的类维生素A形式，支持细胞可能使用细胞视黄醇结合蛋白将视黄醇从基底室转移到近腔室。在附睾中，细胞视黄醇结合蛋白定位于近端附睾头主细胞的细胞质和静纤毛中，而细胞视黄酸结合蛋白定位于整个附睾的精子和主细胞的静纤毛中以及远端输精管的上皮细胞中。精子染色强度从始段到尾部逐渐减弱。上皮细胞中存在高水平的细胞视黄醇结合蛋白，附睾头精子中存在高水平的细胞视黄酸结合蛋白，已知参与精子成熟所需因子的合成和分泌，表明维生素A可能在这一过程中发挥作用。
The distribution of cellular retinol‐binding protein (CRBP) and cellular retinoic acid‐binding protein (CRABP) in rat testis and epididymis was examined by the peroxidase‐antiperoxidase immunolocalization technique. In the testis, cellular retinol‐binding protein was localized exclusively in the Sertoli cells. Staining varied with the stages of the seminiferous epithelium cycle and was maximal prior to the maturation divisions. Cellular retinoic acid‐binding protein was localized exclusively in the germinal cells in the adluminal compartment. The results suggest that retinoic acid may be the retinoid form used by the germinal cells, and that Sertoli cells may use the cellular retinol‐binding protein to transfer retinol from the basal to the adluminal compartment. In the epididymis, cellular retinol‐binding protein was localized in the cytoplasm and stereocilia of the principal cells in the proximal caput epididymidis, while cellular retinoic acid‐binding protein was localized in the spermatozoa and the stereocilia of the principal cells throughout the epididymis and in the epithelial cells of the distal vas deferens. Sperm staining intensity decreased from the initial segment to the cauda. The presence of high levels of cellular retinol‐binding protein in the epithelial cells and high levels of cellular retinoic acid‐binding protein in the spermatozoa of the caput epididymidis, known to be involved in the synthesis and secretion of factors necessary for sperm maturation, suggests that vitamin A may have a role in this process.