Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
Multianalyte lateral flow immunoassay for simultaneous detection of protein-based inflammation biomarkers and pathogen DNA
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DOI：
10.1016/j.snb.2021.131283
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
Anna Klebes;Anna-Sophia Kittel;R. Verboket;F. von Stetten;Susanna M Früh
中科院分区：
文献类型：
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作者：
Anna Klebes;Anna-Sophia Kittel;R. Verboket;F. von Stetten;Susanna M Früh
一次性检测多种生物分子类别对于许多领域来说都是非常理想的，特别是对于即时诊断。例如，伤口感染是患者健康的一个主要问题，并导致我们的医疗保健系统付出巨大的努力。在这方面，通过核酸分析同时检测病原体和检测局部炎症生物标志物来监测感染伤口是实现个性化治疗、改善临床结果、从而降低总体医疗成本的关键。在这方面，伤口渗出液提供了一种有吸引力的样品材料，可以以非侵入性方式收集。在此，我们报告了一种多分析物检测方法的开发，该方法可在 35 分钟内同时检测一个样本中的炎症生物标志物和病原体 DNA。蛋白质兼容的扩增和标记将核酸信息转化为蛋白质检测的测量原理。与侧流免疫分析快速检测相结合，可以使用相同的分析条件快速、直接地分析多种生物分子类别。为了证明多分析物测定的可行性，使用了促炎细胞因子白细胞介素 6 (IL-6) 和机会性病原体铜绿假单胞菌 (P. aeruginosa) 的 gDNA。 4 ng/mL IL-6 和 70 个拷贝/反应的检测限。铜绿糖 DNA 符合临床相关范围，因此具有改善护理点伤口管理的巨大潜力。
The detection of multiple biomolecule classes in one go is highly desirable for a wide variety of areas, and in particular for point-of-care diagnostics. For example, wound infections are a major problem for patient’s health and cause huge efforts in our healthcare system. In this regard, monitoring infected wounds through simultaneous detection of pathogens via nucleic acid analysis and detection of local inflammation biomarkers is key in order to enable a personalized therapy, improve the clinical outcome and thus, leading to a reduction of overall healthcare costs. In this regard, wound exudate offers an attractive sample material which can be collected in a non-invasive manner. Here, we report the development of a Multianalyte-Assay detecting inflammation biomarkers and pathogen DNA simultaneously from one sample within 35 min. Protein-compatible amplification and labeling transforms nucleic acid information into the measurement principle for protein detection. The combination with rapid detection via lateral flow immunoassay enables a fast and straightforward analysis of multiple biomolecule classes using identical assay conditions. To demonstrate the feasibility of the Multianalyte-Assay, the proinflammatory cytokine interleukin-6 (IL-6) and gDNA of the opportunistic pathogenPseudomonas aeruginosa(P. aeruginosa) are used. The detection limits of 4 ng/mL IL-6 and 70 copies/reactionP. aeruginosagDNA meet the clinically relevant range and thus, having tremendous potential to improve the wound management at the point-of-care.