Effects of nitric oxide on nitrogen metabolism and the salt resistance of rice (Oryza sativa L.) seedlings with different salt tolerances
Effects of nitric oxide on nitrogen metabolism and the salt resistance of rice (Oryza sativa L.) seedlings with different salt tolerances
复制标题
一氧化氮对不同耐盐性水稻幼苗氮代谢及耐盐性的影响
DOI：
10.1016/j.plaphy.2020.06.013
复制
发表时间：
2020-10-01
影响因子：
6.5
通讯作者：
Zhang, Junhua
中科院分区：
文献类型：
--
作者：
Huang, Jie;Zhu, Chunquan;Zhang, Junhua
盐胁迫严重抑制水稻生产力。一氧化氮（NO）是植物中的内源信号分子，可以提高水稻对非生物胁迫的抵抗力。先前的研究还表明，氮代谢对于水稻的胁迫耐受性至关重要。然而，NO影响水稻幼苗氮代谢的生理和分子机制仍不清楚。通过金源85（耐盐）和辽粳763（盐敏感）两个水稻品种的水培试验，探讨NO能否缓解盐胁迫对氮代谢的负面影响，提高水稻幼苗的抗盐性。结果表明：（1）施用NO缓解了盐胁迫对株高和生物量积累的抑制作用，提高了水稻叶片的氮含量。 (2)盐胁迫下金源85和辽粳763的蔗糖和脯氨酸积累显着增加，过氧化物酶活性分别提高107%和67.7%。 (3)NO显着提高了盐胁迫下两个水稻品种谷氨酸脱氢酶、蔗糖合酶和蔗糖磷酸合酶的活性。 (4)此外,NO还调节AMT、NIA和SUT基因的表达水平,但这些调节效果因水稻品种和处理的不同而不同。结果表明，NO主要通过增加谷氨酸脱氢酶和过氧化物酶活性以及蔗糖积累来增强氮代谢和抗氧化能力，缓解盐胁迫对水稻生产性能的负面影响。
Salt stress inhibits rice productivity seriously. Nitric oxide (NO) is an endogenous signaling molecule in plants that can improve the resistance of rice to abiotic stresses. Previous studies also showed that nitrogen metabolism is essential for rice stress-tolerance. However, the physiological and molecular mechanisms by how NO affects the nitrogen metabolisms of rice seedlings remain unclear. A hydroponic experiment with two rice varieties, Jinyuan85 (salt tolerant) and Liaojing763 (salt sensitive), was carried out to explore whether NO could alleviate the negative effects of salt stress on nitrogen metabolism and increase salt resistance of rice seedlings. The results showed that (1) the application of NO alleviated the inhibitory effects of salt stress on plant height and biomass accumulation, and increased the nitrogen content of rice leaf. (2) the accumulation of the sucrose and proline was markedly increased in salt stress after application of NO, and peroxidase activities was increased by 107% and 67.7% for Jinyuan85 and Liaojing763, respectively. (3) NO significantly increased the activities of glutamate dehydrogenase, sucrose synthase and sucrose phosphate synthase in both rice varieties under salt stress. (4) Additionally, NO regulated the expression levels of AMT, NIA and SUT genes, but these regulation effects are different with rice varieties and treatments. The results suggested that NO mainly increased the glutamate dehydrogenase and peroxidase activities and sucrose accumulation to enhance the nitrogen metabolism and antioxidative capacity, and alleviated the negative effects of salt stress on rice performance.