Intracerebral grafting of neuronal cell suspensions. I. Introduction and general methods of preparation.
Intracerebral grafting of neuronal cell suspensions. I. Introduction and general methods of preparation.
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神经细胞悬液的脑内移植。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
F. Gage
中科院分区：
文献类型：
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作者：
A. Björklund;U. Stenevi;R. Schmidt;S. Dunnett;F. Gage
详细描述了以游离细胞悬浮液的形式移植中脑和中隔胚胎组织的步骤。这包括解剖供体胚胎，在胰酶中孵化，机械分离，以及将立体定向注射到成年受体大鼠的大脑中。讨论了其中的一些技术问题和局限性。
The steps involved in the grafting of mesencephalic and septal embryonic tissue in the form of dissociated cell suspensions are described in detail. This includes dissection of the donor embryos, incubation in trypsin, mechanical dissociation, and stereotaxic injection into the brains of adult recipient rats. Some of the technical problems and limitations are discussed.