GHz Ultrasound and Electrode Chip-Scale Arrays Stimulate and Influence Morphology of Human Neural Cells
GHz Ultrasound and Electrode Chip-Scale Arrays Stimulate and Influence Morphology of Human Neural Cells
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DOI：
10.1109/tuffc.2022.3152427
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发表时间：
2022-06-01
影响因子：
3.6
通讯作者：
Lal, Amit
中科院分区：
文献类型：
--
作者：
Balasubramanian, Priya S.;Lal, Amit
本研究描述了芯片级千兆赫（GHz）超声（US）和电刺激对体外神经细胞形态、功能和活力的影响。GHz频率的刺激是使用氮化铝压电换能器上制作的硅晶片上，在1.47 GHz，对应于膜的厚度模式谐振工作。这些装置用于体外刺激SH-SY5Y神经细胞，并观察对受刺激细胞的形态和活力的影响。可以使用这些装置单独递送超声刺激或US刺激结合电刺激。活力测试表明，神经元在较宽的GHz US刺激强度（0 - 1.2 W/cm（2））范围内保持结构完整性和活力，验证了在无毒剂量的US下进行测量。根据先前研究的输出，使用这些设备验证神经刺激，活化细胞的归一化荧光强度在1.9和2.4之间。在三项独立试验中，在1.47 GHz和0.05 W/cm（2）峰值强度下的300 s超声波刺激导致核伸长减少17.5%，横截面积减少17.8%（p <0.01）。在超声波刺激或共刺激下，F-actin控制的细胞长度增加了16.3%（p <0.01）。与对照组相比，超声刺激后神经突长度增加了75.8%（p <0.01）。本文展示了新的GHz US和电芯片级阵列，在神经兴奋和细胞形态方面具有明显的效果。
This study describes the effects of chip-scale gigahertz (GHz) ultrasound (US) and electrical stimulus on the morphology, functionality, and viability of neural cells in vitro. The GHz frequency stimulation is achieved using aluminum nitride piezoelectric transducers fabricated on a silicon wafer, operating at 1.47 GHz, corresponding to the film's thickness mode resonance. These devices are used to stimulate SH-SY5Y neural cells in vitro and observe effects on the morphology and viability of the stimulated cells. It is possible to use these devices to deliver either ultrasonic stimulus alone or US stimulus in conjunction with electrical stimulus. Viability tests demonstrated that the neurons retained structural integrity and viability across a wide range of GHz US stimulus intensities (0-1.2 W/cm(2)), validating that measurements occur at nontoxic doses of US. Neural stimulation is validated with these devices following the outputs of a previous study, with the normalized fluorescence intensity of activated cells between 1.9 and 2.4. The 300-s ultrasonic stimulation at 1.47 GHz and 0.05 W/cm(2) peak intensity led to a decrease in nuclear elongation by 17.5% and a cross-sectional area decrease by 17.8% across three independent trials of over 150 cells per category (p < 0.01). The F-actin governed cellular elongation increased in length by up to 16.3% in cells exposed to an ultrasonic stimulus or costimulus (p < 0.01). Neurite length increased following ultrasonic stimulation compared with control by 75.8% (p < 0.01). This article demonstrates new GHz US and electrical chip-scale arrays with apparent effects in both neural excitation and cell morphology.