Development and Population Results of a Fully Automated Homogeneous Assay for LDL Triglyceride
Development and Population Results of a Fully Automated Homogeneous Assay for LDL Triglyceride
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DOI：
10.1373/jalm.2017.024554
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发表时间：
2018-03-01
影响因子：
2
通讯作者：
Furusyo, Norihiro
中科院分区：
文献类型：
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作者：
Ito, Yasuki;Ohta, Motoko;Furusyo, Norihiro
背景资料：低密度脂蛋白（LDL）是通过其胆固醇含量（LDL-C）来测量的，但有人认为LDL甘油三酯（LDL-TG）也可能与冠状动脉疾病风险有关。LDL-TG可以测量后ultracenthegation或电泳，但这些都是劳动密集型的方法，表明需要一个自动化的均相assays.Methods：TG-丰富的脂蛋白（TRLs），低密度脂蛋白和高密度脂蛋白被分离ultracenthegation和用于确定最佳特性的表面活性剂和各种酶的测定发展。我们分析了测定的精密度和线性，并与超离心后的结果进行了比较。从一个大的人口研究（n = 12284受试者）的血清样本被用来产生LDL-TG的参考区间，并确定在各种类型的lipidemias.Results的水平：LDL-TG的测定已开发通过使用表面活性剂1和2，和酶直接测量LDL-TG的自动分析仪。有一个很好的相关性与此测定和分离后的LDL通过超离心分离的结果。当该测定应用于正常和高脂血症受试者的血清样本时，正常值中位数为0.09 mmol/L，在LDL-C升高、高脂血症、混合型高脂血症和高乳糜微粒血症受试者中观察到的中位显著升高分别为0.19、0.18、0.28和0.43 mmol/L，而这些受试者的平均LDL-C值为2.25，4.01，2.66，3.96，和2.43 mmol/L.Conclusions：我们已经开发了一种自动化的LDL-TG的均相测定在研究和临床实验室的潜在用途，并证明，TG摩尔含量的LDL是约5%的胆固醇含量。
Background: Low-density lipoprotein (LDL) is measured by its cholesterol content (LDL-C), but it has been suggested that LDL triglyceride (LDL-TG) may also be related to coronary artery disease risk. LDL-TG can be measured after ultracentrifugation or electrophoresis, but these are labor intensive methods, indicating the need for an automated homogeneous assay.Methods: TG-rich lipoproteins (TRLs), LDL, and HDL were isolated by ultracentrifugation and used to determine optimal characteristics of surfactants and various enzymes for assay development. We analyzed assay precision and linearity, and compared results with those obtained after ultracentrifugation. Serum samples from a large population study (n = 12284 subjects) were used to generate reference intervals for LDL-TG and to determine levels in various types of hyperlipidemia.Results: An assay for LDL-TG has been developed by use of surfactants 1 and 2, and enzymes to measure LDL-TG directly on an automated analyzer. There was an excellent correlation between results obtained with this assay and after isolation of LDL by ultracentrifugation. When the assay was applied to serum samples from normal and hyperlipidemic subjects, median normal values were 0.09 mmol/L, with significant median elevations observed in subjects with increased LDL-C, hypertriglyceridemia, combined hyperlipidemia, and hyperchylomicronemia of 0.19, 0.18, 0.28, and 0.43 mmol/L, respectively, as compared with mean LDL-C values in these subjects of 2.25,4.01, 2.66, 3.96, and 2.43 mmol/L, respectively.Conclusions: We have developed an automated homogeneous assay for LDL-TG for potential use in research and clinical laboratories, and documented that the TG molar content of LDL is about 5% of its cholesterol content.