BILE-ACID STIMULATION OF COLONIC ADENYLATE-CYCLASE AND SECRETION IN RABBIT
BILE-ACID STIMULATION OF COLONIC ADENYLATE-CYCLASE AND SECRETION IN RABBIT
复制标题
DOI：
10.1007/bf01072128
复制
发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
SCHOENFIELD, LJ
中科院分区：
文献类型：
--
作者：
CONLEY, DR;COYNE, MJ;SCHOENFIELD, LJ
体外研究表明，环腺苷酸（cAMP）系统可能是胆汁酸诱导结肠分泌的介质。本文研究了不同剂量脱氧胆酸（DCA）对家兔结肠腺苷酸环化酶（AC）活性和净分泌的影响。AC随着DCA浓度的增加而显著增加（P < 0.01）;在8 mM时，活性为126 ± 0.01。每分钟6 pmol cAMP/mg蛋白，或对照的4倍。磷酸二酯酶活性（PDE）受影响，仅8 mMDCA就显著降低（P < 0.01）。在4、6和8 mMDCA的富碳酸氢盐溶液中，管腔液体积以分级方式显著增加（P < 0.01）。结肠粘膜cAMP系统的刺激强烈暗示为介导DCA诱导的结肠分泌。
The cyclic AMP (cAMP) system in in vitro studies may be the mediator of bile acid-induced colonic secretion. The in vivo effect of various doses of deoxycholic acid (DCA) on adenylate cyclase activity (AC) and net secretion in the rabbit colon were studied. AC increased significantly (P < 0.01) with each increasing concentration of DCA; at 8 mM the activity was 126 .+-. 6 pmols cAMP/mg protein per min, or 4 times the control. Phosphodiesterase activity (PDE) was affected and significantly decreased (P < 0.01) by only 8 mM DCA. The volume of luminal fluid increased significantly (P < 0.01) as a bicarbonate-rich solution with 4, 6 and 8 mM DCA in graded fashion. Stimulation of a colonic mucosal cAMP system is strongly implicated as mediating DCA-induced colonic secretion.