Arabidopsis COP1-interacting protein 1 is a positive regulator of ABA response
Arabidopsis COP1-interacting protein 1 is a positive regulator of ABA response
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拟南芥 COP1 相互作用蛋白 1 是 ABA 反应的正调节因子
DOI：
10.1016/j.bbrc.2016.06.147
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发表时间：
2016
影响因子：
3.1
通讯作者：
Gong Qingqiu
中科院分区：
文献类型：
--
作者：
Ren Chenxia;Zhu Xili;Zhang Pingping;Gong Qingqiu
COP 1相互作用蛋白1（CIP 1，At 5g 41790）是拟南芥中第一个与COP 1相互作用的蛋白，但其生理功能至今仍不清楚。在这里，我们表明，CIP 1是脱落酸（阿坝）反应的正调节。CIP 1主要在光合细胞和维管组织中表达，其启动子活性可被渗透胁迫和阿坝诱导。CIP 1蛋白定位于质膜。然后对T-DNA插入片段cip 1 - 1进行了表征。该突变体对渗透胁迫敏感，具有阿坝不敏感的表型。RNA测序结果显示cip 1 - 1在非生物胁迫反应中的基因表达水平低于野生型。同时，阿坝生物合成基因的转录水平比野生型高incip 1 - 1。这些结果表明，CIP 1正参与阿坝反应。
COP1-interacting protein 1 (CIP1, At5g41790) was the first reported interacting protein for CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) of Arabidopsis; however its physiological function has remained unknown for two decades. Here we show that CIP1 is a positive regulator of abscisic acid (ABA) response. CIP1 is mainly expressed in the photosynthetic cells and the vascular tissue, and its promoter activity can be induced by osmotic stress and ABA. The CIP1 protein is localized to the plasma membrane. A T-DNA insertion mutantcip1-1was then characterized. The mutant is sensitive to osmotic stress and has ABA insensitive phenotypes. RNA sequencing showed thatcip1-1has lower levels of gene expression in abiotic stress response compared with the wild-type. Meanwhile, transcript levels of ABA biosynthesis genes are higher incip1-1than in the wild-type. These results suggested that CIP1 is positively involved in ABA response.