Influence of hydrochloric acid concentration on the demineralization of cortical bone
Influence of hydrochloric acid concentration on the demineralization of cortical bone
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DOI：
10.1016/j.cherd.2010.04.013
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发表时间：
2011-01-01
影响因子：
3.9
通讯作者：
Figueiredo, Helena
中科院分区：
文献类型：
--
作者：
Figueiredo, Margarida;Cunha, Sara;Figueiredo, Helena
尽管脱矿骨基质被认为是一种成功的移植材料，结合了骨传导和骨诱导特性，但在文献中发表了关于其骨诱导能力的相互矛盾的结果。这可能是以下不同的脱矿程序，自然会导致产品具有不同的properties.本工作探讨了类似的人皮质骨样品使用三种不同浓度的盐酸溶液（0.6 M，1.2 M和2.4 M）的脱矿过程的评价。样品钙含量测定（原子吸收光谱法）在不同的浸泡时间，允许建设相应的动力学曲线。相和化学组成分别通过X射线衍射光谱和傅里叶变换红外分析来实现。结构的修改，其次是光和扫描电子显微镜和定量压汞法（在孔隙率和孔径分布方面）。正如预期的那样，增加酸浓度导致的脱矿率的增加，但不是成比例的方式。然而，发现酸浓度对样品结构特征的最显著影响之一。事实上，对于经受最高盐酸浓度的样品，检测到孔隙率的显著增加。显微镜观察表明，尽管脱矿作用导致结构变形，但基本显微结构得以保留。(C)2010年，化学工程师学会。Elsevier B.V.出版，保留所有权利。
Although demineralized bone matrix has been considered a successful grafting material, combining both osteo-conductive and osteoinductive properties, conflicting results have been published in the literature regarding its bone-inducing abilities. This may be a consequence of following different demineralization procedures that naturally result in products with different properties.The present work examines the evaluation of the demineralization process of similar samples of human cortical bone using three different concentrations of hydrochloric acid solutions (0.6 M, 1.2 M and 2.4 M). Sample calcium content was determined (by Atomic Absorption Spectroscopy) at various immersion times, allowing the construction of the corresponding kinetic profiles. Phase and chemical composition were enabled by X-Ray Diffraction Spectroscopy and Fourier Transform Infrared Analysis, respectively. Structural modifications were followed by Light and Scanning Electron Microscopy and quantified by mercury porosimetry (in terms of porosity and pore size distribution).As expected, increasing the acid concentration led to an increase in the demineralization rate, but not in a proportional way. However, one of the most significant effects of the acid concentration was found on the sample structural features. In fact, a considerable increment in porosity was detected for the sample subjected to the highest hydrochloric acid concentration. Microscopic observations demonstrated that despite the structural deformation resultant from demineralization, the basic microstructure was preserved. (C) 2010 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.