The potential of human peripheral blood derived CD34+cells for ex vivo red blood cell production
The potential of human peripheral blood derived CD34+cells for ex vivo red blood cell production
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DOI：
10.1016/j.jbiotec.2009.08.017
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发表时间：
2009-10-26
影响因子：
4.1
通讯作者：
Al-Rubeai, Mohamed
中科院分区：
文献类型：
--
作者：
Boehm, Daniela;Murphy, William G.;Al-Rubeai, Mohamed
采用一种新的每日传代策略，在无基质培养系统中研究了外周血来源的CD34+细胞体外红系生成的潜力。通过将外周血来源的CD34+细胞扩增至1.5x10(6)倍，该方法获得了以前仅报道的来自更强大的干细胞来源的CD34+细胞的扩增倍数，如脐带血、骨髓和动员的外周血细胞。细胞表面标记分析显示，未成熟的CD34+细胞分化为80%的CD71-/GPA+细胞和高达45%的去核细胞，表明有大量的终末成熟。细胞拥挤对体外红细胞生成有决定性的影响。在指数生长期，单位表面积的细胞密度而不是每介质体积的细胞浓度决定了细胞的扩张，在指数生长期间，越拥挤的细胞表现出总体扩张的减少。然而，在红细胞生成的晚期，当细胞不再增殖时，增加细胞密度可以增强细胞的活力。这些结果表明，外周血来源的造血干细胞在扩增潜力方面可以替代骨髓、脐带血或白细胞来源的细胞。这在研究放大和成熟的条件方面提供了明显的优势，并可能在未来有特殊的临床应用。(C)2009爱思唯尔B.V.保留所有权利。
The potential of peripheral blood derived CD34+ cells for ex vivo erythropoiesis was investigated in a stroma-free culture system using a novel strategy of daily passaging. By expanding PB-derived CD34+ cells up to 1.5 x 10(6)-fold this method achieved expansion factors previously only reported for CD34+ cells derived from more potent stem cell sources such as cord blood, bone marrow and mobilized peripheral blood. Analysis of cell surface markers showed differentiation of immature CD34+ cells to populations with 80% CD71-/GpA+ cells and up to 45% enucleated cells, indicating a significant amount of terminal maturation. Cell crowdedness was found to have decisive effects on in vitro erythropoiesis. Cell density per surface area rather than cell concentration per media volume determined cell expansion during exponential growth where more crowded cells showed reduced overall expansion. In late stage erythropoiesis, however, when cells no longer proliferating, increased cell density was seen to enhance cell viability. These results indicate that peripheral blood derived haematopoietic stem cells can be an alternative to cells sourced from bone marrow, cord blood or leukapheresis in terms of expansion potential. This provides distinct advantages in terms of availability for studies of conditions for scale-up and maturation, and may have particular clinical applications in the future. (C) 2009 Elsevier B.V. All rights reserved.