Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)
Identification and complete genome characterization of a novel picornavirus in turkey (Meleagris gallopavo)
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DOI：
10.1099/vir.0.043224-0
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发表时间：
2012-10-01
影响因子：
3.8
通讯作者：
Reuter, Gabor
中科院分区：
文献类型：
--
作者：
Boros, Akos;Nemes, Csaba;Reuter, Gabor
小核糖核酸病毒科的成员是人类和动物的重要病原体，尽管与数千种已知的鸟类物种（> 10000）相比，只有少数（n=11）小核糖核酸病毒已从鸟类来源中鉴定出来。本研究报告的宏基因组检测和完整的基因组特征的一种新的火鸡小核糖核酸病毒的粪便样本收集在匈牙利的8个火鸡农场。使用RT-PCR，无论是健康的（三分之二）和受影响的（八分之七）与肠道和/或发育迟缓综合征的商业火鸡被证明是脱落病毒在七（88%）的八个农场。病毒基因组序列（turkey/M176/2011/匈奴; Gen Bank登录号JQ 691613）显示与最近在美国火鸡和鸡中报道的小核糖核酸病毒的部分P3基因组区域具有高度的氨基酸序列同一性（96%），可能属于同一物种。在P1和P2区域，turkey/M176/2011/匈奴;与kobuviruses和turdivirus 1最密切相关，但不同。全基因组分析显示存在特征性小核糖核酸病毒氨基酸基序、潜在的II型样5' UTR内部核糖体进入位点（在禽类来源的小核糖核酸病毒中首次鉴定）以及在火鸡/M176/2011/匈奴和小核糖核酸病毒属禽肝病毒和Kobuvirus的成员的3' UTR处发现的保守的48 nt长的“杠铃样”结构。火鸡小核糖核酸病毒-一种新的小核糖核酸病毒物种-在匈牙利和美国健康和受影响火鸡的粪便样本中普遍存在，表明该病毒在火鸡农场中在全球范围内发生和流行。需要进一步的研究来调查这种小核糖核酸病毒在食用动物中的病原学作用和致病潜力。
Members of the family Picornaviridae are important pathogens of humans and animals, although compared with the thousands of known bird species (>10 000), only a few (n=11) picornaviruses have been identified from avian sources. This study reports the metagenomic detection and complete genome characterization of a novel turkey picornavirus from faecal samples collected from eight turkey farms in Hungary. Using RT-PCR, both healthy (two of three) and affected (seven of eight) commercial turkeys with enteric and/or stunting syndrome were shown to be shedding viruses in seven (88%) of the eight farms. The viral genome sequence (turkey/M176/2011/HUN; Gen Bank accession no. JQ691613) shows a high degree of amino acid sequence identity (96%) to the partial P3 genome region of a picornavirus reported recently in turkey and chickens from the USA and probably belongs to the same species. In the P1 and P2 regions, turkey/M176/2011/HUN; is related most closely to, but distinct from, the kobuviruses and turdivirus 1. Complete genome analysis revealed the presence of characteristic picornaviral amino acid motifs, a potential type II-like 5' UTR internal ribosome entry site (first identified among avian-origin picornaviruses) and a conserved, 48 nt long 'barbell-like' structure found at the 3' UTR of turkey/M176/2011/HUN and members of the picornavirus genera Avihepatovirus and Kobuvirus. The general presence of turkey picornavirus - a novel picornavirus species - in faecal samples from healthy and affected turkeys in Hungary and in the USA suggests the worldwide occurrence and endemic circulation of this virus in turkey farms. Further studies are needed to investigate the aetiological role and pathogenic potential of this picornavirus in food animals.