Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.
Coated vesicles transport newly synthesized membrane glycoproteins from endoplasmic reticulum to plasma membrane in two successive stages.
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包被的囊泡分两个连续阶段将新合成的膜糖蛋白从内质网转运到质膜。
DOI：
10.1073/pnas.77.2.780
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发表时间：
1980
影响因子：
11.1
通讯作者：
R. Fine
中科院分区：
文献类型：
--
作者：
J. Rothman;R. Fine
水泡性口炎病毒的G蛋白是一种跨膜糖蛋白，其从粗面内质网中的合成位点通过高尔基体转运到质膜。网格蛋白包被的囊泡已从感染水泡性口炎病毒的CHO细胞中纯化，并显示含有与网格蛋白接近化学计量的G蛋白。脉冲追踪实验表明，这种G蛋白是一种过境形式，并揭示了G被运送到细胞表面的两个连续波的包被囊泡。在早期波中携带的G1蛋白的寡糖是“高甘露糖”种类的，其可以被内切糖苷酶H切割;在第二、后期波中的G2蛋白的寡糖对内切糖苷酶H具有抗性。因此，早期的波被认为是对应于G蛋白在包被囊泡中从内质网到高尔基体的运输，在高尔基体中寡糖被加工并对内切糖苷酶H产生抗性;随后的波代表从高尔基体到质膜的运输。
The G protein of vesicular stomatitis virus is a transmembrane glycoprotein that is transported from its site of synthesis in the rough endoplasmic reticulum to the plasma membrane via the Golgi apparatus. Clathrin-coated vesicles have been purified from CHO cells infected with vesicular stomatitis virus and shown to contain G protein in amounts nearly stoichiometric with clathrin. Pulse-chase experiments have demonstrated that this G protein is a transit form and have revealed that G is transported to the cell surface in two successive waves of coated vesicles. The oligosaccharides of G1 protein carried in the early wave are of the "high-mannose" variety which can be cleaved by the enzyme endoglycosidase H; the oligosaccharides of G2 protein in the second, later wave are resistant to endoglycosidase H. The early wave is therefore proposed to correspond to transport of G protein in coated vesicles from the endoplasmic reticulum to the Golgi apparatus, where the oligosaccharides are processed and resistance to endoglycosidase H is conferred; the succeeding wave would represent transport from the Golgi apparatus to the plasma membrane.