TRANSCRIPTIONAL CONTROL OF HERPESVIRUS GENE-EXPRESSION - GENE FUNCTIONS REQUIRED FOR POSITIVE AND NEGATIVE REGULATION
TRANSCRIPTIONAL CONTROL OF HERPESVIRUS GENE-EXPRESSION - GENE FUNCTIONS REQUIRED FOR POSITIVE AND NEGATIVE REGULATION
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DOI：
10.1073/pnas.83.2.256
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发表时间：
1986-01-01
影响因子：
11.1
通讯作者：
KNIPE, DM
中科院分区：
文献类型：
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作者：
GODOWSKI, PJ;KNIPE, DM
我们已经使用了体外核径流测定，以测量特定的单纯疱疹病毒基因的转录水平在不同的时间在裂解感染。我们分析了DNA复制的抑制和两种单纯疱疹病毒调节蛋白的缺陷对这些基因转录的影响。我们提供的证据表明。alpha。ICP4基因在溶菌感染过程中负调控。ICP4基因转录的调控需要。蛋白质ICP8（其中ICP =感染细胞多肽）。转录。ICP8,可以利用。1ICP5和。2个糖蛋白C （gC）基因依赖于ICP4。当DNA复制被阻断时，2gC基因被强烈抑制。ICP8缺陷也导致亲本病毒基因组中ICP4、ICP8、ICP5和gC基因的转录水平升高。我们的研究结果表明，ICP8可能在维持病毒基因表达的高度有序级联中起重要作用。
We have used an in vitro nuclear run-off assay to measure the levels of transcription of specific herpes simplex virus genes at different times during a lytic infection. We analyzed the effects of inhibition of DNA replication and of defects in two herpes simplex virus regulatory proteins on the transcription of these genes. We present evidence that the transcription of the .alpha.ICP4 gene is negatively regulated during a lytic infection. The regulation of ICP4 gene transcription requires the .beta. protein ICP8 (where ICP = infected cell polypeptide). Transcription of the .beta.ICP8, .gamma.1ICP5, and .gamma.2 glycoprotein C (gC) genes was dependent on ICP4, and transcription of the .gamma.2gC gene was strongly inhibited when DNA replication was blocked. Defects in ICP8 also resulted in increased levels of transcription of the ICP4, ICP8, ICP5, and gC genes from parental viral genomes. Our results suggest that ICP8 may be important in maintaining the highly ordered cascade of viral gene expression.