Beta-chemokines in nasal secretions of infants with respiratory syncytial virus-induced respiratory infections
Beta-chemokines in nasal secretions of infants with respiratory syncytial virus-induced respiratory infections
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DOI：
10.1089/088318701753436880
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发表时间：
2001-01-01
期刊：
影响因子：
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通讯作者：
Welliver, Robert C.
中科院分区：
文献类型：
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作者：
Garofalo, Roberto P.;Olszewska-Pazdrak, Barbara;Welliver, Robert C.
免疫机制被认为有助于毛细支气管炎的发病机制，但关键途径的性质尚不清楚。本研究的目的是确定由于呼吸道合胞病毒（RSV）感染引起的各种疾病的婴儿中巨噬细胞炎症蛋白1 α (MIP1alpha)， eotaxin和表达调控的正常t细胞表达和分泌（RANTES）的量是否不同。对56例因RSV感染而单独患有上呼吸道疾病（URI） （n=25）或下呼吸道疾病（n=31）的婴儿和未满13月龄的儿童采集鼻咽分泌物（NPS）样本。17名无症状婴儿作为对照。对数据进行对数变换后，采用t检验比较各组分泌物中趋化因子的数量。在RSV感染时，无论是否存在喘息，所有三种趋化因子的数量都增加。然而，喘息婴儿的mip -1 α浓度显著高于单纯URI患儿（p=0.039）。低氧喘息患儿的mip -1 α (p=0.029)和eotaxin （p=0.017）均显著高于无低氧喘息患儿，且与个体血氧饱和度呈负相关。最后，在呼吸道症状改善后至少2至3周，所有三种趋化因子在分泌物中仍以高水平存在。这些发现支持了mip -1 α可能在RSV感染时诱导炎症和喘息中发挥作用的假设。
Immune mechanisms are believed to contribute to the pathogenesis of bronchiolitis, but the nature of the critical pathways is unknown. The purpose of this study was to determine if quantities of the beta-chemokines macrophage inflammatory protein 1 alpha (MIP1alpha), eotaxin, and regulated on expression, normal T-cell expressed and secreted (RANTES) were different among infants with various forms of illness due to respiratory syncytial virus (RSV) infection. Samples of nasopharyngeal secretions (NPS) were obtained from 56 infants and children less than 13 months of age with either upper respiratory illness (URI) alone (n=25) or lower respiratory tract illness (n=31) due to RSV infection. Seventeen asymptomatic infants served as controls. Quantities of chemokines in secretions were compared between groups using the t-test after logarithmic transformation of data. Quantities of all three chemokines were increased at the time of RSV infection regardless of whether wheezing was present. However, concentrations of MIP-1alpha were significantly greater (p=0.039) in wheezing infants than in those with URI alone. Quantities of MIP-1alpha (p=0.029) and eotaxin (p=0.017) were each significantly greater in wheezing infants with hypoxia than wheezing infants without hypoxia, and were inversely related to individual values of oxygen saturation. Finally, all three chemokines were still present at high levels in secretions for at least 2 to 3 weeks after respiratory symptoms had improved. These findings permit the hypothesis that MIP-1alpha may possibly play a role in the induction of inflammation and wheezing at the time of RSV infection.