Engulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans
Engulfment genes cooperate with ced-3 to promote cell death in Caenorhabditis elegans
复制标题
DOI：
10.1038/35084103
复制
发表时间：
2001-07-12
期刊：
影响因子：
64.8
通讯作者：
Schnabel, R
中科院分区：
文献类型：
--
作者：
Hoeppner, DJ;Hengartner, MO;Schnabel, R
遗传学研究已确定了在线虫秀丽隐杆线虫 (1-3) 中十多个参与程序性细胞死亡（细胞凋亡）功能的基因。尽管每个细胞死亡基因的突变对细胞存活或吞噬的最终影响已被广泛描述，但人们对这些突变如何影响死亡和吞噬的动力学或这两个过程之间的相互作用知之甚少。我们使用四维诺马斯基延时视频显微镜来详细跟踪细胞死亡基因如何调节早期秀丽隐杆线虫胚胎中凋亡细胞死亡和去除的程度和动力学。在这里，我们表明，当细胞受到微弱的促凋亡信号时，阻断吞噬可以增强细胞的存活率。因此，介导尸体清除的基因也可以发挥主动杀死细胞的作用。
Genetic studies have identified over a dozen genes that function in programmed cell death (apoptosis) in the nematode Caenorhabditis elegans(1-3). Although the ultimate effects on cell survival or engulfment of mutations in each cell death gene have been extensively described, much less is known about how these mutations affect the kinetics of death and engulfment, or the interactions between these two processes. We have used four-dimensional-Nomarski time-lapse video microscopy to follow in detail how cell death genes regulate the extent and kinetics of apoptotic cell death and removal in the early C. elegans embryo. Here we show that blocking engulfment enhances cell survival when cells are subjected to weak pro-apoptotic signals. Thus, genes that mediate corpse removal can also function to actively kill cells.