DNA restriction fragment length variability in Diospyros kaki and related Diospyros species

DNA restriction fragment length variability in Diospyros kaki and related Diospyros species
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柿树和相关柿树种的 DNA 限制性片段长度变异性

DOI:
10.21273/hortsci.29.7.809
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发表时间:
1994
期刊:
影响因子:
1.9
通讯作者:
S. Kobayashi
S. Kobayashi
中科院分区:
农林科学4区
文献类型:
--
作者:
Yuri Nakamura;S. Kobayashi

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对11个日本柿品种的线粒体DNA和叶绿体DNA进行了限制性片段长度分析。以及其他五种柿属植物。总基因组DNA经7种限制性内切酶酶切、Southern印迹杂交后,与5种mtDNA探针(油菜Pst Ⅰ或SalI片段)杂交。mtDNA)和一个cpDNA探针[烟草(Nicotiana tabacum(L.)cpDNA]。所有日本柿品种都显示出相同的mtDNA和cpDNA片段模式,而使用mtDNA和cpDNA探针观察到物种间的多态性。
Restriction fragment length analyses of mitochondrial DNA (mtDNA) and chloroplast DNA (cpDNA) were carried out on 11 cultivars of Japanese persimmon (Diospyros kaki Thunb.) and five other Diospyros species. Total genomic DNA was digested with seven restriction endonucleases, Southern blotted, and hybridized with five mtDNA probes (PstI or SalI fragments of Brassica campestris L. mtDNA) and one cpDNA probe [pTBa1, BamHI fragment of Nicotiana tabacum (L.) cpDNA]. All Japanese persimmon cultivars displayed identical mtDNA and cpDNA fragment patterns, while polymorphisms among species were observed using both mtDNA and cpDNA probes [...]
Honda, H.:“一种使用非放射性 rDNA 作为探针来鉴定杂交体的简单而有效的方法。”
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