A 24-HOUR PERIODICITY IN THE LH-RELEASE APPARATUS OF FEMALE RATS, DISCLOSED BY BARBITURATE SEDATION
A 24-HOUR PERIODICITY IN THE LH-RELEASE APPARATUS OF FEMALE RATS, DISCLOSED BY BARBITURATE SEDATION
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DOI：
10.1210/endo-47-3-198
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发表时间：
1950-01-01
期刊：
影响因子：
4.8
通讯作者：
SAWYER, CH
中科院分区：
文献类型：
--
作者：
EVERETT, JW;SAWYER, CH
使用二苯胺和阿托品的早期研究表明，在作者所在群体中的4天周期大鼠中，一种对排卵分泌黄体生成素必不可少的特定神经源性刺激，在发情前期的下午2点至4点之间激活脑垂体。有24小时的证据。最近还报道了促黄体生成素释放装置神经成分的兴奋性节律。目前的结果用不同的方法证实了前人的结论。戊巴比妥钠标准剂量30毫克/公斤，经皮注射。在发情前期下午2点给药，可防止脑垂体排卵激活。当连续的下午在相同的关键时间重复使用Nembutal治疗时，Graafian毛囊持续2-3天的结果。在第2天和第3天进行补充注射。要求在下午3：30-4：00进行。在这3天中的任何一天，如果不进行治疗或将治疗推迟到下午4点以后，脑下垂体将迅速激活，并在当晚排卵。阿米替林、Dial、巴比妥和苯巴比妥也有类似的结果。连续几天使用巴比妥类药物阻断黄体生成素的释放，可以发现卵泡周期在下一次发情前期2天左右闭锁时终止。考虑到这种卵泡周期与“自发持续发情”和“轻度发情”中发现的重叠周期之间的异同。24小时。节律，虽然可能是其他自发排卵形式以及大鼠的促黄体生成素释放机制的一种特性，但在对各种动物进行研究之前，不能被认为是普遍的特征。
Earlier investigation by the use of Dibenamine and atropine indicated that in 4-day cyclic rats in the authors'' colony, a specific neuro-genic stimulus, essential for the ovulatory discharge of LH, activates the hypophysis between 2 and 4 P.M. on the day of proestrus. Evidence of a 24-hr. rhythm of excitability in the neural component of the LH-release apparatus was also recently reported. The present results confirm the earlier ones by different methods. Nembutal (pentobarbital Na[long dash]standard dose 30 mg./kg., intraperit.) prevents the ovulatory activation of the hypophysis when administered at 2 P.M. during proestrus. Persistence of graafian follicles for 2-3 days results when Nembutal treatment is repeated on successive afternoons at the same critical hours. On the 2nd and 3rd days a supplementary injn. is required at 3:30-4 P.M. If, on any of these 3 days, treatment is omitted or postponed until after 4 P.M., pituitary activation will occur promptly and ovulation will follow during that night. Similar results are produced by amytal, dial, barbital and phenobarbital. Blockade of LH release by treatment with the barbiturates on successive days discloses a follicular cycle which terminates in atresia about 2 days before onset of the next proestrus.. Consideration is given to similarities and differences between such follicular cycles and the overlapping ones found in "spontaneous persistent estrus" and "light estrus." The 24-hr. rhythm, although possibly a property of the LH-release mechanism in other spontaneously ovulating forms as well as the rat, cannot be considered as a universal characteristic until a variety of animals has been investigated.