Cloning and analysis of the locus for mosquito vitellogenic carboxypeptidase.
Cloning and analysis of the locus for mosquito vitellogenic carboxypeptidase.
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DOI：
10.1111/j.1365-2583.1994.tb00140.x
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发表时间：
1993-01-01
影响因子：
2.6
通讯作者：
Raikhel, A.S.
中科院分区：
文献类型：
--
作者：
Deitsch, K.W.;Raikhel, A.S.
卵黄生成羧肽酶是一种53 kDa的卵黄蛋白，由雌性蚊子埃及伊蚊的脂肪体响应血餐而产生。其表达具有性别、阶段和组织特异性，与主要卵黄蛋白卵黄原蛋白相同。该基因是无内含子的，两个等位基因已被克隆和测序，包括编码区两侧超过1.5 kb。最近确定为节肢动物发起人的capsite共识是目前在转录的起始位点。该帽位点的上游是重复四次的16 bp不完全回文，显示出与定义的激素反应元件的强同源性。此外，还发现了一个与果蝇蛋黄蛋白基因中的脂肪体增强子和双性结合位点非常相似的区域，以及几个潜在的脂肪体特异性调节蛋白结合位点。
Vitellogenic carboxypeptidase is a 53 kDa yolk protein produced by the fat body of the female mosquito,Aedes aegypti, in response to a blood meal. Its expression is sex‐, stage‐ and tissue‐specific and is identical to that of the major yoik protein, vitellogenin. The gene is intronless and two alleles have been cloned and sequenced, including more than 1.5 kb on both sides of the coding region. A capsite consensus recently identified as an arthropod initiator is present at the start site of transcription. Upstream of this capsite is a 16 bp imperfect palindrome repeated four times showing strong homology to defined hormone‐response elements. In addition, a region that closely resembles the fat body enhancer and double sex binding site from theDrosophilayolk protein genes and several potential fat body‐specific regulatory protein binding sites were found.