KINETICS OF ENTRY OF PROTEINS INTO THE MYELIN MEMBRANE 1
KINETICS OF ENTRY OF PROTEINS INTO THE MYELIN MEMBRANE 1
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蛋白质进入髓磷脂膜的动力学 1
DOI：
10.1111/j.1471-4159.1978.tb00150.x
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发表时间：
1978
影响因子：
4.7
通讯作者：
J. Hazlett
中科院分区：
文献类型：
--
作者：
J. Benjamins;Roderick Iwata;J. Hazlett
17天龄大鼠脑切片，用[3H]甘氨酸或[3H]‐亮氨酸孵育以标记蛋白质。从切片中分离髓磷脂，在含有十二烷基硫酸钠的缓冲液中用不连续凝胶电泳分离蛋白质。放射性碱性蛋白和沃尔夫克蛋白以相似的初始速率出现在髓磷脂中，它们的进入在15 - 120分钟之间几乎呈线性，没有可检测到的滞后。放射性蛋白脂蛋白在0 - 30分钟内以碱性蛋白和沃尔夫克蛋白的四分之一的速度出现在髓磷脂中，然后在45 - 120分钟内以与其他蛋白相当的速度进入髓磷脂。
Brain slices were prepared from 17‐day old rats, and incubated with [3H]glycine or [3H]‐leucine to label proteins. Myelin was isolated from the slices, and the proteins were separated by discontinuous gel electrophoresis in buffers containing sodium dodecyl sulfate. Radioactive basic and Wolfgram proteins appeared in myelin at similar initial rates, and their entry was nearly linear between 15 and 120 min with no detectable lag. Radioactive proteolipid protein appeared in myelin at one‐fourth the rate of the basic and Wolfgram proteins between 0 and 30 min, then entered at a rate comparable to the other proteins between 45 and 120 min.