The Evolution of Polystyrene as a Cell Culture Material.
The Evolution of Polystyrene as a Cell Culture Material.
复制标题
DOI：
10.1089/ten.teb.2018.0056
复制
发表时间：
2018-10
期刊：
影响因子：
--
通讯作者：
Fisher JP
中科院分区：
文献类型：
--
作者：
Lerman MJ;Lembong J;Muramoto S;Gillen G;Fisher JP
聚苯乙烯（PS）将体外细胞培养从其卑微的开端带到了现代，推动了数十个研究领域的发展。本文综述了培养材料的材料、制作和处理方法的发展。然而，天然PS表面在体外很难促进细胞粘附和生长。为了克服这一点，液体表面沉积，高能等离子体活化和新兴的功能化方法改变了表面化学。这篇评论旨在强调许多潜在的应用，第一个广泛接受的聚合物生长表面。虽然大多数体外研究都是在二维表面上进行的，但三维（3D）培养模型的重要性不容忽视。将PS过渡到专门的3D培养表面的方法也进行了审查。具体而言，突出了铸造，静电纺丝，3D打印和微载体方法将PS转移到3D培养表面。该材料的广泛应用使其不可能突出每一种用途，但目标仍然是展示其作为通用和定制细胞培养表面的多功能性和潜力。审查的结论与新兴的支架方法，并根据研究结果，提出了我们的见解，未来的步骤PS作为一个组织培养平台。
Polystyrene (PS) has brought in vitro cell culture from its humble beginnings to the modern era, propelling dozens of research fields along the way. This review discusses the development of the material, fabrication, and treatment approaches to create the culture material. However, native PS surfaces poorly facilitate cell adhesion and growth in vitro. To overcome this, liquid surface deposition, energetic plasma activation, and emerging functionalization methods transform the surface chemistry. This review seeks to highlight the many potential applications of the first widely accepted polymer growth surface. Although the majority of in vitro research occurs on two-dimensional surfaces, the importance of three-dimensional (3D) culture models cannot be overlooked. The methods to transition PS to specialized 3D culture surfaces are also reviewed. Specifically, casting, electrospinning, 3D printing, and microcarrier approaches to shift PS to a 3D culture surface are highlighted. The breadth of applications of the material makes it impossible to highlight every use, but the aim remains to demonstrate the versatility and potential as both a general and custom cell culture surface. The review concludes with emerging scaffolding approaches and, based on the findings, presents our insights on the future steps for PS as a tissue culture platform.