Cytoplasmic components in hepatic cell lysosomes.
Cytoplasmic components in hepatic cell lysosomes.
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DOI：
10.1083/jcb.12.1.198
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发表时间：
1962-01
影响因子：
7.8
通讯作者：
PORTER, K R
中科院分区：
文献类型：
--
作者：
ASHFORD, T P;PORTER, K R
几年前，De Duve和他的同事基于对分离细胞质颗粒的生化分析，提出了溶酶体的概念(1)。他报告说，许多水解酶集中在由小的亚细胞单位组成的部分中，这些亚细胞单位沉积在通常的线粒体和微粒体部分之间。在这一发现之后，其中一些特性在肝管周围致密体中得到证实，Essner和Novikoff(2)利用电子显微镜发现，酸性磷酸酶的酶产物只存在于这些致密体中。他们还发现，人肝细胞中的多种色素颗粒既具有致密体的精细结构特征，又具有溶酶体的酸性磷酸酶活性(3)。在本报告中，我们将把通常出现在肝细胞胆道周围（或管周）位置的多形致密体称为溶酶体。这与现在细胞学家的惯常做法是一致的。诚然，如果进行平行的细胞化学研究以确定酸性磷酸酶和其他酸性水解酶的存在与否，则鉴定将更加明确。
De Duve and his colleagues, a few years ago, originated the concept of lysosomes based on the biochemical analysis of isolated cytoplasmic particles (1). He reported that a number of hydrolytic enzymes are concentrated in a fraction consisting of small subcellular units which sediments between the usual mitochondrial and microsomal fractions. Subsequent to this discovery, some of these properties have been demonstrated in hepatic pericanalicular dense bodies, and Essner and Novikoff (2), using the electron microscope, have shown that the enzyme product of acid phosphatase is found exclusively in these bodies. They have also found that a variety of pigment granules in human liver cells possess both the fine structure characteristic of dense bodies and the acid phosphatase activity of lysosomes (3). In this report we shall refer to polymorphic dense bodies which appear normally in peribiliary (or pericanalicular) positions in liver cells as lysosomes. This is in keeping with what is now the usual practice among cytologists. Admittedly the identification would be more definite if parallel cytochemical studies had been done to determine the presence or absence of acid phosphatase and other acid hydrolases.