Labeling primary nerve stem cells with quantum dots.
Labeling primary nerve stem cells with quantum dots.
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DOI：
10.1166/jnn.2011.5322
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发表时间：
2011-11
影响因子：
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通讯作者：
Jing Zhang-;X. Lv;Xing Jia;Yu-lin Deng;H. Qing;Hai‐Yan Xie
中科院分区：
文献类型：
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作者：
Jing Zhang-;X. Lv;Xing Jia;Yu-lin Deng;H. Qing;Hai‐Yan Xie
被动负载羧基量子点标记原代神经干细胞。量子点对神经干细胞作用的研究表明，在一定浓度下，量子点在一周内对神经干细胞的生长和增殖影响不大，对神经干细胞的分化潜能及神经元和星形胶质细胞的蛋白表达均无影响。结果表明，该标记方法适用于体外标记研究。结合量子点独特的光学性质，有可能在体内实现神经干细胞的命运追踪。
The primary nerve stem cells (NSCs) were labeled with the carboxyl QDs by passive loading. The studies on QDs' effect on the NSCs showed that with a proper concentration, QDs have little effect on NSCs growth and proliferation within a week, and the QDs did not affect either differentiation potential of NSCs or the protein expression of neuron and astrocyte derived from NSCs. The results suggested that this labeling method is appropriate for labeling studies in vitro. Combined with the unique optical properties of QDs, it is possibly to fulfill NSCs fate-tracking in vivo.