In Situ Fluorescence Imaging of Myelination
In Situ Fluorescence Imaging of Myelination
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DOI：
10.1369/jhc.2010.954842
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发表时间：
2010-07-01
影响因子：
3.2
通讯作者：
Wang, Yanming
中科院分区：
文献类型：
--
作者：
Wang, Changning;Popescu, Daniela C.;Wang, Yanming
我们描述了一种新的荧光染料，3-（4-氨基苯基）-2H-苯并吡喃-2-酮（称为髓鞘化合物或CMC），可用于在脊椎动物神经系统中的髓鞘的原位荧光成像。当通过尾静脉注射给药时，CMC选择性地染色中枢神经系统（CNS）和外周神经系统（PNS）中的大束有髓纤维。在CNS中，CMC容易进入大脑并选择性地定位于有髓鞘区域，如胼胝体和小脑。CMC还选择性地染色PNS中的有髓神经。CMC在高髓鞘小鼠模型中的染色模式与免疫组织化学染色一致。与免疫组织化学染色相似，CMC选择性结合于存在于白色物质束中的髓鞘。与CMC不同，髓鞘碱性蛋白的常规抗体染色也染色纹状体中的少突胶质细胞细胞质以及小脑中的颗粒层。CMC的体内应用也通过PNS中有髓神经的荧光成像来证明。（J Histochem Cytochem 58：611-621，2010）
We describe a novel fluorescent dye, 3-(4-aminophenyl)-2H-chromen-2-one (termed case myelin compound or CMC), that can be used for in situ fluorescent imaging of myelin in the vertebrate nervous system. When administered via intravenous injection into the tail vein, CMC selectively stained large bundles of myelinated fibers in both the central nervous system (CNS) and the peripheral nervous system (PNS). In the CNS, CMC readily entered the brain and selectively localized in myelinated regions such as the corpus callosum and cerebellum. CMC also selectively stained myelinated nerves in the PNS. The staining patterns of CMC in a hypermyelinated mouse model were consistent with immunohistochemical staining. Similar to immunohistochemical staining, CMC selectively bound to myelin sheaths present in the white matter tracts. Unlike CMC, conventional antibody staining for myelin basic protein also stained oligodendrocyte cytoplasm in the striatum as well as granule layers in the cerebellum. In vivo application of CMC was also demonstrated by fluorescence imaging of myelinated nerves in the PNS. (J Histochem Cytochem 58:611-621, 2010)