Buckling Nanoneedle for Characterizing Single Cells Mechanics Inside Environmental SEM
Buckling Nanoneedle for Characterizing Single Cells Mechanics Inside Environmental SEM
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DOI：
10.1109/tnano.2009.2036997
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发表时间：
2011-03-01
影响因子：
2.4
通讯作者：
Fukuda, Toshio
中科院分区：
文献类型：
--
作者：
Ahmad, Mohd Ridzuan;Nakajima, Masahiro;Fukuda, Toshio
我们提出了一种屈曲纳米针作为力传感器来表征单细胞的刚度。屈曲纳米针很容易通过使用聚焦离子束蚀刻从商业化的原子力显微镜悬臂制造。使用屈曲纳米针进行单细胞刚度表征具有显著的优势。首先，可以防止由于过大的压入力造成的严重的电池损坏。第二，单细胞刚度性质的大变化可以容易地从压痕后细胞表面上的凹痕标记和/或通过比较压痕期间纳米针的屈曲长度来检测。屈曲纳米针的校准进行了实验和数值。两种方法的校准结果显示出良好的一致性。校准数据显示了压痕力和纳米针的屈曲长度之间的关系。这种关系被用于在屈曲纳米针和单细胞之间的纳米压痕实验期间获得力数据。我们通过使用集成SEM（ESEM）-纳米操纵器系统内部的屈曲纳米针对单个W303野生型酵母细胞的机械性能进行了原位测量。比较了单胞在不同压力和不同温度范围内的局部刚度特性。这种单细胞刚度变化的检测方法可以应用于未来基于单细胞刚度分析的快速疾病诊断。
We propose a buckling nanoneedle as a force sensor for stiffness characterization of single cells. The buckling nanoneedle was easily fabricated by using focused ion beam etching from a commercialized atomic force microscope cantilever. There are notable advantages of using buckling nanoneedle for single cells stiffness characterizations. First, severe cell damage from an excessive indentation force could be prevented. Second, large variations in single cells stiffness property could be easily detected either from the dented mark on the cell surface after the indentation and/or by comparing the buckling length of the nanoneedle during the indentation. The calibrations of the buckling nanoneedle were done experimentally and numerically. The calibration results from both methods showed a good agreement. The calibration data show the relationship between the indentation force and the buckling length of the nanoneedle. This relationship was used for obtaining force data during a nanoindentation experiment between a buckling nanoneedle and single cells. We performed in situ measurements of mechanical properties of individual W303 wildtype yeast cells by using a buckling nanoneedle inside an integrated SEM (ESEM)-nanomanipulator system. Finer local stiffness property of single cells was compared at different pressure and different temperature ranges. This detection method of the stiffness variations of the single cells could be applied in the future fast disease diagnosis based on single-cell stiffness analysis.