ULTRASTRUCTURE OF ULTRAVIOLET RADIATION-INDUCED HAIRLESS MOUSE SKIN CARCINOGENESIS, WITH SPECIAL REFERENCE TO THE EPIDERMAL-DERMAL JUNCTION
ULTRASTRUCTURE OF ULTRAVIOLET RADIATION-INDUCED HAIRLESS MOUSE SKIN CARCINOGENESIS, WITH SPECIAL REFERENCE TO THE EPIDERMAL-DERMAL JUNCTION
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DOI：
10.3109/00313028609059487
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发表时间：
1986-07-01
期刊：
影响因子：
4.5
通讯作者：
WILKINSON, F
中科院分区：
文献类型：
--
作者：
CANFIELD, PJ;XU, FN;WILKINSON, F
紫外线（UV）诱导的皮肤病理学的超微结构进行了研究，以补充以前报道的肉眼和光镜下的结果，并进一步评估的有用性的动物模型的研究阳光相关的表皮肿瘤在man. Hairless白化病（HRA/Skh-1）小鼠暴露于最小的辐射剂量从一个过滤的光源发射UVA和UVB，接近太阳辐射。正常和增生性皮肤，带蒂乳头状瘤，原位癌和浸润性鳞状细胞癌的样品进行处理，一旦他们的身份被证实通过光学显微镜检查的透射电子显微镜。随着恶性肿瘤的发展，角质形成细胞的多形性变得更加明显，细胞与细胞的接触减少。对于乳头状瘤、原位癌和浸润性鳞状细胞癌，表皮连接的进行性破坏在坦率的侵袭后变得明显。因此，各种病理变化之间的大多数差异不是绝对的，而是程度上的，支持浸润性鳞状细胞癌代表恶性肿瘤的终末期的概念，其可能从头产生，直接来自增生性皮肤，或来自其他肿瘤类型。小鼠肿瘤结构与人类类似肿瘤的相似性支持了动物模型的有用性，并表明该结果对人类与阳光相关的肿瘤具有影响。
The ultrastructure of ultraviolet (UV)-induced skin pathology was studied in mice to complement previously reported gross and light microscopic findings, and to assess further the usefulness of the animal model for study of sunlight associated epidermal tumours in man. Hairless albino (HRA/Skh-1) mice were exposed to a minimal erythemal dose from a filtered light source emitting both UVA and UVB, approximating solar emission. Samples of normal and hyperplastic skin, pedunculated papillomas, carcinomas in situ and invasive squamous cell carcinomas were processed for transmission electron microscopy once their identity was confirmed by light microscopic examination. Keratinocyte pleomorphism became more marked and cell to cell contact diminished as malignancy developed. For papillomas, carcinomas in situ and invasive squamous cell carcinomas, there was a progressive disruption of the epidermal junction which became marked upon frank invasion. Most of the differences between the various categories of pathological change, therefore, were not absolute but rather of degree, supporting the notion that invasive squamous cell carcinoma represents an end stage for malignancy which may arise de novo, directly from hyperplastic skin, or proceed from other tumour types. The similarity in structure of the mouse tumours to comparable tumours in man supports the usefulness of the animal model and suggests that the results have implications for sunlight associated tumours in man.