beta-Naphthoflavone- and self-induced metabolism of 3,3',4,4'-tetrachlorobiphenyl in hepatic microsomes of the male, pregnant female and foetal rat.
beta-Naphthoflavone- and self-induced metabolism of 3,3',4,4'-tetrachlorobiphenyl in hepatic microsomes of the male, pregnant female and foetal rat.
复制标题
雄性、怀孕雌性和胎鼠肝微粒体中 3,3,4,4-四氯联苯的 β-萘黄酮和自身诱导代谢。
DOI：
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
A. Brouwer
中科院分区：
文献类型：
--
作者：
D. Morse;P. J. Bladeren;E. Wehler;A. Brouwer
1. The in vitro metabolism of 3,3',4,4'-tetrachloro-[14C]-biphenyl ([14C]-TCB) by hepatic microsomes from the Wistar rat was investigated with liver microsomes from the male, pregnant female and foetus. 2. Three hydroxylated metabolites (4-OH-3,3',4,5'-tetrachlorobiphenyl, 5-OH-3,3',4,4'-tetrachlorobiphenyl, and 6-OH-3,3',4,4'-tetrachlorobiphenyl) were identified by hplc and gc-ms after incubations of liver microsomes from the beta-naphthoflavone-pretreated male rat and TCB-treated pregnant rat. No metabolites of [14C]-TCB were found after incubation with foetal liver microsomes from dams pretreated with [14C]-TCB. The results indicate that the in vivo accumulation of 4-OH-tetraCB in the foetal compartment is probably due to transplacental transport rather than the formation of this metabolite in the foetus. 3. Pretreatment of the male rat with beta-naphthoflavone substantially induced the formation of hydroxylated metabolites, but pretreatment with phenobarbital and dexamethasone was without effect. Based on in vitro incubations of liver microsomes from the beta-naphthoflavone pretreated male rat, an apparent Km and Vmax of 4.5 microM and 240 pmol/mg protein/min respectively was determined for the metabolism of [14C]-TCB. The formation of phenolic metabolites of [14C]-TCB was most likely dependent on P4501A induction.
鉴定大鼠肝脏中大环内酯类抗生素诱导的细胞色素 P-450 为糖皮质激素反应性细胞色素 P-450p。
影响因子：
2.9
作者：
Wrighton,SA;Maurel,P;Schuetz,EG;Watkins,PB;Young,B;Guzelian,PS
通讯作者：
Guzelian,PS
影响因子：
5.1
作者：
LANS, MC;KLASSONWEHLER, E;BROUWER, A
通讯作者：
BROUWER, A