Production of succinate from glucose, cellobiose, and various cellulosic materials by the ruminal anaerobic bacteria Fibrobacter succinogenes and Ruminococcus flavefaciens
Production of succinate from glucose, cellobiose, and various cellulosic materials by the ruminal anaerobic bacteria Fibrobacter succinogenes and Ruminococcus flavefaciens
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DOI：
10.1007/bf02785981
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发表时间：
1997-10-01
影响因子：
3
通讯作者：
Eriksson, KEL
中科院分区：
文献类型：
--
作者：
Gokarn, RR;Eiteman, MA;Eriksson, KEL
以葡萄糖、纤维二糖、微晶纤维素、Walseth纤维素（酸溶胀纤维素）、纸浆和汽爆白杨为底物，比较了产琥珀酸纤维杆菌（Fibrobacter succinogenes S85）和产黄瘤胃球菌（Ruminococcus flavefaciens FD-1）产有机酸的能力。F.生产的主要最终产品。来自这些底物中的每一种的琥珀酸酯是琥珀酸酯（69.5-83%），主要的次级产物是乙酸酯（16-30.5%）。最大琥珀酸生产力范围从14.1 mg/L. h的蒸汽爆破黄色白杨纸浆纸59.7 mg/L. h。对于R.来自纤维二糖、微晶纤维素和酸溶胀Walseth纤维素的主要终产物flavefaciens是乙酸酯（39-46%），制浆纸和蒸汽爆破的黄色白杨产生琥珀酸酯（42-54%）作为主要产物。最大琥珀酸生产力的R。产黄杆菌的范围从纤维二糖的9.21 mg/L. h到制浆纸的43.1 mg/L. h。一般而言，在较低的最大生产力下，R. flavefaciens比F.在相似的发酵条件下，这两种生物体的最大琥珀酸生产率与先前报道的在葡萄糖和玉米浆上生长的产琥珀酸安氏杆菌的59 mg/L. h的值相当。
The production of organic acids by two anaerobic ruminal bacteria, Fibrobacter succinogenes S85 and Ruminococcus flavefaciens FD-1, was compared with glucose, cellobiose, microcrystalline cellulose, Walseth cellulose (acid swollen cellulose), pulped paper, and steam-exploded yellow poplar as substrates. The major end product produced by F. succinogenes from each of these substrates was succinate (69.5-83%), the principal secondary product was acetate (16-30.5%). Maximum succinate productivity ranged from 14.1 mg/L.h for steam-exploded yellow Poplar to 59.7 mg/L.h for pulped paper. For R. flavefaciens, the major end product from cellobiose, microcrystalline cellulose, and acid-swollen Walseth cellulose was acetate (39-46%), pulped paper and steam-exploded yellow poplar yielded succinate (42-54%) as the major product. Maximum succinate productivity by R. flavefaciens ranged from 9.21 mg/L.h for cellobiose to 43.1 mg/L.h for pulped paper. In general, much less succinate was produced at a lower maximum productivity by R. flavefaciens than by F. succinogenes under similar fermentation conditions. The maximum succinate productivities by these two organisms are comparable to the previously reported value of 59 mg/L.h for Anderobiospirillum succiniciproducens grown on glucose and corn steep liquor.