Magnetic resonance angiography and vascular corrosion casting as tools in biomedical research: application to transgenic mice modeling Alzheimer's disease
Magnetic resonance angiography and vascular corrosion casting as tools in biomedical research: application to transgenic mice modeling Alzheimer's disease
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DOI：
10.1179/016164104225016281
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发表时间：
2004-07-01
影响因子：
1.9
通讯作者：
Beckmann, N
中科院分区：
文献类型：
--
作者：
Krucker, T;Schuler, A;Beckmann, N
体内成像技术目前在生物医学和制药研究领域受到相当大的关注。主要的成像模态之一是磁共振成像（MRI）。MRI的多参数性质使得能够以高空间分辨率从完整生物体非侵入性地获得解剖、功能甚至分子信息。在这里，我们描述了使用一种MRI模式，即血管造影（MRA），非侵入性研究的动脉血管结构的APP 23转基因小鼠阿尔茨海默病模型。由于该技术的空间分辨率有限，因此使用血管腐蚀铸造对血管系统进行了强大的分析，从而补充了体内研究。这两种技术都揭示了这些小鼠的年龄依赖性血流改变和脑血管异常。我们的经验表明，MRA辅以铸型分析是描述血管改变和测试AD动物模型中新治疗概念的重要工具。此外，由于是非侵入性的，MRA也可以应用于患有这种疾病的患者的研究。
In vivo imaging technologies are presently receiving considerable attention in the biomedical and pharmaceutical research areas. One of the principal imaging modalities is magnetic resonance imaging (MRI). The multiparametric nature of MRI enables anatomical, functional and even molecular information to be obtained non-invasively from intact organisms at high spatial resolution. Here we describe the use of one MRI modality, namely angiography (MRA), to non-invasively study the arterial vascular architecture of APP23 transgenic mice modeling Alzheimer's disease. Because the spatial resolution of the technique is limited, the in vivo studies are complemented by a powerful analysis of the vasculature using vascular corrosion casting. Both techniques revealed age-dependent blood flow alterations and cerebrovascular abnormalities in these mice. Our experience suggests that MRA complemented by cast analysis are important tools to describe vascular alterations and test new therapy concepts in animal models of AD. Furthermore, being non-invasive, MRA can also be applied to studies in patients suffering from this disease.