Norovirus detection from sera of young children with acute norovirus gastroenteritis
Norovirus detection from sera of young children with acute norovirus gastroenteritis
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DOI：
10.1016/j.jcv.2016.03.021
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发表时间：
2016-06-01
影响因子：
8.8
通讯作者：
Vesikari, Timo
中科院分区：
文献类型：
--
作者：
Huhti, Leena;Hemming-Harlo, Maria;Vesikari, Timo
背景资料：抗原血症和病毒血症是常见的轮状病毒感染，但只有少数研究表明诺如病毒（NoV）RNA在血液circulation.Objectives：检测诺如病毒RNA从血清中的诺如病毒感染的儿童和研究，如果诺如病毒RNAemia与临床严重程度的急性胃肠炎。优化半巢式PCR检测血清中的NoV衣壳RNA。结果：176例血清标本中有11例（6.3%）检出NoV RNA。在8例病例中发现了NoV GII.4，在1例病例中发现了GII.3、GII.6和GII.7。在所有病例中，血清中检测到的基因型与粪便中的结果相同。血清中的大多数NoV RNA检测发生在年龄小于18个月的幼儿中。结论：血清中的NoV RNA是一种罕见的发现，仅限于年幼的儿童。(C)© 2016 Elsevier B.V.版权所有。
Background: Antigenemia and viremia are common in rotavirus infection but only few studies have shown norovirus (NoV) RNA in the blood circulation.Objectives: To detect NoV RNA from serum of NoV-infected children and study if NoV RNAemia correlates with clinical severity of acute gastroenteritis.Study design: Serum specimens were collected from 176 Finnish children with acute NoV gastroenteritis. Semi-nested PCR was optimized to detect NoV capsid RNA from sera. NoV positive samples were further analyzed by sequencing.Results: NoV RNA was found in 11/176 (6.3%) of serum specimens. NoV GII.4 was found in 8 cases and GII.3, GII.6 and GII.7 in one case each. The genotypes detected in serum were identical to findings in stools in all cases. Most of the NoV RNA detections in serum were in young children less than 18 months of age. The clinical features of NoV serum-positive cases were not different from NoV serum-negative cases.Conclusion: NoV RNA in serum is an uncommon finding limited to young children. (C) 2016 Elsevier B.V. All rights reserved.