The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells.
The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cells.
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PGC-1α对恶性子宫内膜癌细胞增殖及能量代谢的影响
DOI：
10.2147/ott.s79960
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发表时间：
2015
影响因子：
4
通讯作者：
Ma X
中科院分区：
文献类型：
--
作者：
Ren Z;Yang H;Wang C;Ma X
众所周知，过氧化体增殖物激活受体-1α(PGC-1α)在组织能量代谢中起重要作用。然而，pGC-1α在恶性子宫内膜癌中的作用仍不清楚。收集子宫内膜癌组织40例，增生期子宫内膜组织15例，正常子宫内膜组织21例。实时荧光定量聚合酶链式反应检测前列腺素C-1α和雌激素相关受体γ(ERR-γ)基因表达水平。采用酶联免疫吸附试验检测丙酮酸激酶和异柠檬酸脱氢酶的浓度。结果采用医学统计学方法进行分析。子宫内膜癌组织和增生性子宫内膜组织中PGC-1α和ERRγ的表达水平均显著高于正常子宫内膜组织。合并2型糖尿病的子宫内膜癌患者PGC-1α和ERRγ的表达水平均高于无糖尿病患者。子宫内膜腺癌组织中PGC-1α和ERRγ的表达水平随临床分期、肌层侵犯深度和转移淋巴结数目的增加而升高。子宫内膜癌组织中PGC-1α基因表达水平与ERRγ水平呈正相关。子宫内膜癌组织中PGC-1α的表达水平与丙酮酸激酶和异柠檬酸脱氢酶的浓度呈正相关，ERRγ的表达水平也与之呈正相关。提示PGC-1α和ERRγ在子宫内膜癌中的表达上调可能是癌细胞能量代谢的需要，参与了子宫内膜癌的发生发展。
It is well known that peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) plays an important role in tissue energy metabolism. However, the roles of PGC-1α in malignant endometrial cancer remain unknown. Forty cases of endometrial carcinoma, 15 cases with proliferative endometrial tissues, and 21 cases with normal endometrial tissues were collected. Real-time polymerase chain reaction was used to detect the mRNA levels of PGC-1α and estrogen-related receptor gamma (ERRγ). ELISA (enzyme-linked immunosorbent assay) was used to detect the concentrations of pyruvate kinase and isocitrate dehydrogenase. The results were analyzed using medical statistical methods. The mRNA levels of PGC-1α and ERRγ in the endometrial carcinoma tissues and hyperplasic endometrial tissues were significantly greater than those in the normal endometria. The mRNA levels of PGC-1α and ERRγ in the endometrial carcinoma patients with type 2 diabetes were higher than those in patients without diabetes. The mRNA levels of PGC-1α and ERRγ in the endometrial adenocarcinomas increased with clinical staging, depth of myometrial invasion, and increases in the number of metastatic lymph nodes. The PGC-1α mRNA level was positively correlated with ERRγ in the endometrial carcinoma tissues. The mRNA levels of PGC-1α were positively correlated with the concentrations of pyruvate kinase and isocitrate dehydrogenase in the endometrial carcinoma tissues, and similar results were found for ERRγ. Our results suggested that the upregulation of PGC-1α and ERRγ in endometrial cancer might be a requirement for cancer cell energy metabolism, which contributes to the development of endometrial cancer.